Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Derivation and functional annotation of human hematopoietic subpopulations from single-cell DNA methylation data

ABSTRACT: Derivation and functional annotation of human hematopoietic subpopulations from single-cell DNA methylation data

PROVIDER: PRJNA418579 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Derivation and functional annotation of hematopoietic subpopulations from single-cell DNA methylation data

Project description:We report the development of a bisulfite based whole genome protocol suitable for use on single index sorted primary mammalian cells. Application of this protocol to murine and human hematopoietic stem cells provided quantitative DNA methylation measurements of up to 5.7 million CpGs in a single cell. To analyze the resulting datasets we developed a novel analytical approach designed to detect differences at single CpG resolution and identified epigenetically distinct subpopulations within the highly purified and functionally defined stem and progenitor populations. In silico merging of the methylation states from the single cells residing in each subpopulation allowed us to ascribe functional pathways to these epigenetically distinct subpopulations on the basis of their unique epigenetic states.

2017-01-01 | GSE89545 | GEO

Derivation and functional annotation of human hematopoietic subpopulations from single-cell DNA methylation data

2018-04-01 | GSE106957 | GEO

Mus musculus

Project description:Derivation and functional annotation of hematopoietic subpopulations from single-cell DNA methylation data

| PRJNA353010 | ENA

Derivation of functional oocytes from granulosa cells

Project description:Derivation of functional oocytes from granulosa cells

| PRJNA525610 | ENA

Proteome in human hematopoietic stem and progenitor cell subpopulations from polycythemia vera patients and controls

Project description:Polycythemia vera (PV) is a myeloproliferative neoplasm (MPN) characterized by hyper-proliferation of the erythroid, megakaryocytic and granulocytic lineages and the presence of an activating mutation in JAK2. To elucidate mechanisms that regulate PV stem cells, we applied a newly developed data-independent acquisition (DIA) mass spectrometry (MS) technology to purified hematopoietic stem and progenitor cell (HSPC) subpopulations of patients with chronic and progressed PV. Proteomic analyses were supplemented by RNA-sequencing (RNA-seq) and identified targets validated by flow cytometry and functional in vitro assays.

2022-01-19 | PXD021178 | Pride

Functional Annotation of the Bovine Genome

Project description:Functional Annotation of the Bovine Genome

| PRJEB55828 | ENA

Functional annotation of transcriptional factors transactivation domains

Project description:Functional annotation of transcriptional factors transactivation domains

| PRJNA471260 | ENA

Toward a functional annotation of the human genome using artificial transcription factors

Project description:Toward a functional annotation of the human genome using artificial transcription factors.

2010-06-05 | E-GEOD-677 | biostudies-arrayexpress

Toward a functional annotation of the human genome using artificial transcription factors

Project description:Toward a functional annotation of the human genome using artificial transcription factors. Keywords: other

2003-12-12 | GSE677 | GEO

Gene expression profiles of normal mouse mammary cell subpopulations FACS sorted based on expression of biomarkers

Project description:To delineate epithelial subpopulations in mouse mammary tissue, hematopoietic and endothelial cells were depleted from freshly isolated cell suspensions derived from mammary glands using fluorescence-activated cell sorting. The resultant Lin- population was fractionated into four distinct subpopulations using antibodies against CD29, CD24 and CD61. Based on the immunohistochemical phenotype, and in vivo and in vitro functional assays, these subpopulations were identified as fibroblast-containing stromal (CD29loCD24-), mammary stem cell (MaSC)-enriched (CD29hiCD24+CD61+), luminal progenitor (CD29loCD24+CD61+), and mature luminal (CD29loCD24+CD61-) cell subpopulations. Microarray profiling was used to derive gene expression signatures of these 4 subpopulations. The four mammary cell subpopulations were found to have distinct gene expression profiles. Four mammary cell subpopulations from 3-5 pooled mouse tissues were analysed. MS is for the MaSC-enriched cell subpopulation. LP is for the Luminal Progenitor subpopulation. ML is for the Mature Luminal subpopulation.

2010-03-20 | E-GEOD-19446 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data