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Isolation of A Unique Hepatic Stellate Cell Population Expressing Integrin a8 from Embryonic Mouse Livers

ABSTRACT: Isolation of A Unique Hepatic Stellate Cell Population Expressing Integrin a8 from Embryonic Mouse Livers

PROVIDER: PRJNA419875 | ENA |

REPOSITORIES: ENA

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Isolation of A Unique Hepatic Stellate Cell Population Expressing Integrin a8 from Embryonic Mouse Livers

Project description:There are a few markers for embryonic hepatic stellate cells in mouse embryonic livers To identify genes expressed in embryonic hepatic stellate cells, we separated ALCAM+, ALCAM+ PDPN+, and ALCAM+ PDPN- cells from E12.5 mouse livers and analyzed these cells by microarray analysis. After finding expression of Itga8 in ALCAM+ PDPN- cells, we further separated ALCAM+ ITGA8- and ALCAM+ ITGA8+ cells and analyzed by microarray.

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Isolation of a unique hepatic stellate cell population expressing integrin ?8 from embryonic mouse livers.

Project description:BACKGROUND:Hepatic stellate cells (HSCs) play an important role in liver fibrogenesis. However, little is known about their phenotype and role in liver development. The aim of this study is to identify specific markers for embryonic HSCs. RESULTS:Using antibodies against ALCAM and PDPN, we separated mesothelial cells (MCs) and HSCs from developing livers and identified integrin ?8 (ITGA8) as a marker for embryonic desmin+ HSCs that are preferentially localized near the developing liver surface and ?-smooth muscle actin+ perivascular mesenchymal cells around the vein. A cell lineage-tracing study revealed that upon differentiation, MC-derived HSCs or perivascular mesenchymal cells express ITGA8 during liver development. Using anti-ITGA8 antibodies, we succeeded in isolating MC-derived HSCs and perivascular mesenchymal cells from embryonic livers. In direct co-culture, ITGA8+ mesenchymal cells promoted the expression of hepatocyte and cholangiocyte markers in hepatoblasts. In the normal adult liver, expression of ITGA8 was restricted to portal fibroblasts in the portal triad. Upon liver injury, myofibroblasts increased the expression of ITGA8. CONCLUSIONS:ITGA8 is a specific cell surface marker of MC-derived HSCs and perivascular mesenchymal cells in the developing liver. Our data suggest that ITGA8+ mesenchymal cells maintain the phenotype of hepatoblast in liver development. Developmental Dynamics 247:867-881, 2018. © 2018 Wiley Periodicals, Inc.

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