Project description:MyD88 may play a direct role in STING-dependent signaling, or alternatively that STING-dependent pro-inflammatory cytokines may require downstream MyD88-dependent signaling to exert their effect. To determine this, we treated STING or MyD88-deficient murine embryonic fibroblasts (MEFs), bone marrow derived macrophages (BMDM) or dendritic cells (BMDC) with exogenous CDN’s or cytosolic dsDNA (ISD) which triggers STING-signaling and type I IFN production.
Project description:To identify the potential genes that regulated by KDM5B, we performed the CUT&Tag in murine primary BMDMs stimulated with LPS for 1h or not, and analyzed the precipitated DNA with deep sequencing.
Project description:We used RNA-seq to profile the effect of a bacterially derived fatty on LPS-stimulated murine macrophages. From the transcriptional response, there was a significant decrease of inflammatory signals with lipid treatment, and pathway analysis of differentially expressed genes suggests PPAR alpha as potential anti-inflammatory mechanism.
Project description:Dendritic cells isolated from murine bone marrow were cultured for 7 days and then stimulated with 50 ng/mL LPS and 5 mM ATP. Cells derived from both wild type and microRNA-155 knock-out mice. Total RNA was isolated from the cells following stimulation.