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Paenibacillus larvae subsp. larvae DSM 25719

ABSTRACT: Paenibacillus larvae subsp. larvae DSM 25719 Genome sequencing and assembly

PROVIDER: PRJNA42203 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ADFW01.dat.gz	Other
	ADFW01.fasta.gz	Fasta.gz
	ADFW01.master.dat	Other
	ADFW01.dat.gz	Other

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Paenibacillus larvae exoproteins of ERIC I-IV genotypes

Project description:The following strains of Paenibacillus larvae classified as ERIC I-IV genotypes (pl1-pl4) were used in the study: ERIC I - DSM-7030; ERIC II - DSM-25430; ERIC III - LMG-16252; and ERIC IV - DSM-3615. The 7 biological replicates (a-g) of the exoprotein fractions prepared from each ERIC genotype were used in the nanoLC-MS/MS analysis. All 28 samples were consecutively analyzed using an OrbitrapTM FusionTM Tribrid q-OT-IT mass spectrometer. To improve the protein identification and easy exclude contaminants from the medium, we considered the proteins that could originate from the yeast extract and Mueller-Hinton broth (contains beef fusion and casein peptone-acidic hydrolysate) in analyzing the MS data. Thus, we included the sequences related to Saccharomyces cerevisiae and Bos taurus, respectively, to the list of contaminants in MaxQuant. The contaminants.fasta file is provided. Futhermore, we provide the database (uniprot-paenibacillus+larvae.fasta) used for the identification of P. larvae proteins created of different strains of P. larvae. The sequences were downloaded of UniProt on 12.03.2019. Finally, the compressed (zipped) folder "combined" is provided. The data were analyzed in MaxQuant v1.6.3.4.

2019-03-27 | MSV000083636 | MassIVE

Paenibacillus larvae subsp. larvae strain:PFR-Pl-2006

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Paenibacillus larvae subsp. larvae strain:ERICV | isolate:ERICV

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The overall response of Paenibacillus larvae to bodily fluids of honeybee larvae (Apis mellifera).

Project description:Paenibacillus larvae, the causal agent of American Foulbrood disease (AFB), affects honeybee health worldwide. The present study investigates the transcriptional response of this Gram-positive, endospore-forming bacterium to bodily fluids from honeybee larvae. Four different conditions were evaluated with a loop design: sampling of in vitro grown P. larvae cultures one or four hours after addition of larval fluids or BHIT-broth (C1, T1, C4, T4).

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Transcriptome analysis of collagen-adhered S. gallolyticus subsp. gallolyticus [DSM 16831]

Project description:Streptococcus gallolyticus subsp. gallolyticus is a commensal of the human gastrointestinal tract and a pathogen of infective endocarditis and other biofilm-associated infections with exposed collagen. Therefore, this study focuses on the characterization of the biofilm formation and collagen adhesion of S. gallolyticus subsp. gallolyticus under different conditions. It has been observed that lysozyme triggers biofilm formation divergently in the analyzed S. gallolyticus subsp. gallolyticus strains. The transcriptome analysis was performed for two strains which form more biofilm in the presence of lysozyme. Lysozyme leads to higher expression of genes of transcription and translation, of the dlt operon (cell wall modification), of hydrogen peroxide resistance proteins and of two immunity proteins which could be involved in biofilm formation. Furthermore, the adhesion ability of 73 different S. gallolyticus subsp. gallolyticus strains to collagen type I and IV was analyzed. High adhesion ability was observed for the strain UCN 34, whereas the strain DSM 16831 adhered only marginally to collagen. The full genome microarray analysis revealed strain-dependent gene expression due to adhesion. The expression of genes of a transposon and a phage region in strain DSM 16831 were increased, which corresponds to lateral gene transfer. Adherence to collagen leads to a change in the expression of genes of nutrients uptake in the strain UCN 34.

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