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Global transcriptomic response of modified Bacillus anthracis over expressing protective antigen

ABSTRACT: Global transcriptomic response of modified Bacillus anthracis over expressing protective antigen

PROVIDER: PRJNA429267 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6454700.fastq.gz	Fastqsanger.gz
	SRR6454710.fastq.gz	Fastqsanger.gz
	SRR6454701.fastq.gz	Fastqsanger.gz
	SRR6454711.fastq.gz	Fastqsanger.gz
	SRR6454702.fastq.gz	Fastqsanger.gz

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Global transcriptomic response of modified Bacillus anthracis over expressing protective antigen

Project description:In this study we focused on understanding the effect of over expression of recombinant protective antigen (rPA) on the Bacillus anthracis metabolism and genes expression with the purpose of improving the production of this recombinant toxin in particular and recombinant proteins in general. To achieve this goal controlled growth of the B. anthracis ames strain transformed with pYS5 (PA producer) plasmid encoding protective antigen and the corresponding empty vector pSW4 (non-producer) were performed and protein production, bacterial growth characteristics and gene transcription pattern were analyzed. Controlled condition batch runs were performed for both strains and samples from early log (OD 3), log (OD 10) and late log (OD 16) phase were used for gene expression profiling. We found that most of the genes belonging to transport, TCA, glycolysis, PPP and amino acid biosynthesis were up-regulated in the lag phase samples which help the cell coping with the increased requirements of nutrient and energy for rPA expression. These pathways are down-regulated in the log and latelog phase as cellular stress response onsets, which is reflected in the decreased specific growth rate.

2018-11-06 | GSE108973 | GEO

Activity of the Bacillus anthracis 20 kDa protective antigen component

Project description:Edema factor of anthrax binds to the protective antigen (PA83) which cleaves to PA63 and PA20. The role of PA20 was examined using highthroughput gene expression analysis of human peripheral blood mononuclear cells (PBMC) exposed to PA20. Keywords: Toxicity determination PBMC obtained from different individuals over 6 monthes were incubated with and without rPA20

2008-08-26 | E-GEOD-12533 | biostudies-arrayexpress

Activity of the Bacillus anthracis 20 kDa protective antigen component

2008-08-26 | GSE12533 | GEO

Effect of over expressing protective antigen on global gene transcription in Bacillus anthracis BH500.

Project description:Protective antigen (PA) of Bacillus anthracis is being considered as a vaccine candidate against anthrax and its production has been explored in several heterologous host systems. Since the systems tested introduced adverse issues such as inclusion body formation and endotoxin contamination, the production from B. anthracis is considered as a preferred method. The present study examines the effect of PA expression on the metabolism of B. anthracis producing strain, BH500, by comparing it with a control strain carrying an empty plasmid. The strains were grown in a bioreactor and RNA-seq analysis of the producing and non-producing strain was conducted. Among the observed differences, the strain expressing rPA had increased transcription of sigL, the gene encoding RNA polymerase σ54, sigB, the general stress transcription factor gene and its regulators rsbW and rsbV, as well as the global regulatory repressor ctsR. There were also decreased expression of intracellular heat stress related genes such as groL, groES, hslO, dnaJ, and dnaK and increased expression of extracellular chaperons csaA and prsA2. Also, major central metabolism genes belonging to TCA, glycolysis, PPP, and amino acids biosynthesis were up-regulated in the PA-producing strain during the lag phase and down-regulated in the log and late-log phases, which was associated with decreased specific growth rates. The information obtained from this study may guide genetic modification of B. anthracis to improve PA production.

| S-EPMC6208434 | biostudies-literature

Transcription profiling of Bacillus anthracis over 8 hours covering the entire life cycle

Project description:This is a series of expression measurements done during an 8 hour time course of B. anthracis growth in modified G medium. This time frame includes an entire life cycle.

2006-09-01 | E-MEXP-788 | biostudies-arrayexpress

Transcription profiling of murine macrophage-like RAW 264.7 cells treated with EdTx, protective antigen, or LPS for different length of time to examine the effect of EdTx on global transcriptional profiles.

Project description:Edema toxin (EdTx), which is a combination of edema factor and a binding moiety (protective antigen), is produced by Bacillus anthracis, the etiological agent of anthrax. EdTx is an adenylyl cyclase enzyme that converts adenosine triphosphate to adenosine-3',5'-monophosphate, resulting in interstitial edema seen in anthrax patients. We used GeneChip analysis to examine global transcriptional profiles of EdTx-treated RAW 264.7 murine macrophage-like cells at 3 and 6 hr. Experiment Overall Design: RAW 264.7 cells were treated with EdTx (2.5 µg/ml of protective antigen and 0.625 µg/ml of Edema factor), PA (2.5 µg/ml), or LPS (1 ng/mL) for 0, 3, and 6 hr. Each experiment was performed in triplicate, generating a total of 21 arrays (biological replciates).

2007-11-22 | E-GEOD-4712 | biostudies-arrayexpress

Transcriptional Response of Mycobacterium tuberculosis to Arabinomannan-specific Immune Sera

Project description:Transcriptional profiling of Mycobacterium tuberculosis H37Rv exposed to immune sera from C57/BL6 mice immunized with a conjugate of Bacillus anthracis protective antigen and arabinomannan vs. protective antigen alone

2017-03-09 | GSE77711 | GEO

Bacillus anthracis Stern 34F2 sporulation

Project description:Timecourse from log phase growth to sporulation in Bacillus anthracis Stern 34F2 Keywords: time-course

2004-01-08 | GSE840 | GEO

Laboratory adaptation of Bacillus anthracis

Project description:The spore forming pathogen Bacillus anthracis is the etiologic agent of anthrax in humans and animals. It cycles through infected hosts as vegetative cells and is eventually introduced into the environment where it generates an endospore resistant to many harsh conditions. The endospores are subsequently ingested by the next host to begin the next cycle. Outbreaks of anthrax occur regularly worldwide in wildlife and livestock, and the potential for human infection exists whenever humans encounter infected animals. It is also possible to encounter intentional releases of anthrax spores, as was the case in October 2001. Consequently, it is important to be able to rapidly establish the provenance of infectious strains of B. anthracis. Here, we compare protein expression in seven low-passage wild isolates and four laboratory strains of B. anthracis grown under identical conditions using LC-MS/MS proteomic analysis. Of the 1,023 total identified proteins, 96 had significant abundance differences between wild and laboratory strains. Of those, 28 proteins directly related to sporulation were upregulated in wild isolates, with expression driven by Spo0A, CodY, and AbrB/ScoC. In addition, we observed evidence of changes in cell division and fatty acid biosynthesis between the two classes of strains, despite being grown under identical experimental conditions. These results suggest wild B. anthracis cells are more highly tuned to sporulate than their laboratory cousins, and this difference should be exploited as a method to differentiate between laboratory adapted cultures and low passage wild strains isolated during an anthrax outbreak. This knowledge should distinguish between intentional releases and exposure to strains in nature providing a basis for the type of response by public health officials and investigators.

2018-12-04 | PXD010120 | Pride

Identification of Bacillus anthracis genes differentially expressed in human serum

Project description:Bacillus anthracis causes anthrax infections in mammals. Large-scale mortality resulting from the intentional release of B. anthracis spores represents a potential bioterrorism threat. Inhalational anthrax almost invariably proceeds to fatal systemic infection, characterized by massive bacteremia. A better understanding of host-pathogen interactions is urgently needed for effective treatment of this lethal disease. However, virulence mechanisms used by B. anthracis to survive and multiply in human blood are not completely understood. Identification of genes that are differentially expressed during the growth of B. anthracis in human serum can elucidate how this pathogen successfully colonizes the bloodstream. We compared the transcriptional profile of B. anthracis growing in heat-inactivated human serum to that in LB medium. Genes involved in the biosynthesis of purines, certain amino acids and riboflavin and lipid metabolism, genes encoding ABC transporters, respiratory enzymes and several genes with hypothetical function were identified as being upregulated during growth in serum.

2022-12-01 | GSE97567 | GEO

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