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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult HITS-CLIP]

ABSTRACT: Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult HITS-CLIP]

PROVIDER: PRJNA429344 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6456422.fastq.gz	Fastqsanger.gz
	SRR6456423.fastq.gz	Fastqsanger.gz
	SRR6456424.fastq.gz	Fastqsanger.gz

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult HITS-CLIP]

Project description:Dazl (deleted in azoospermia like) is a member of the DAZ family of germ cell-restricted RNA binding proteins required for gametogenesis from worm to human. The direct RNA targets and functions of these essential proteins are poorly understood. Here, we generated high-resolution, transcriptome-wide maps of Dazl-RNA interactions in mouse testes. These maps provide important insights into the mechanism of Dazl recruitment to mRNA and reveal Dazl binding to thousands of mRNAs predominantly through sequence-specific interactions near the polyA tail. Using transgenic mice and fluorescence activated cell sorting (FACS), we isolated DAZL knockout germ cells and used RNA-Seq to identify mRNAs sensitive to DAZL-ablation. Intersecting the RNA-Seq and Dazl-RNA interaction datasets revealed that Dazl enhances expression of a subset of directly-bound transcripts, namely mRNAs for a network of essential cell cycle regulatory genes. Collectively, our integrative analysis delineates a Dazl-dependent post-transcriptional gene regulatory program essential for mammalian germ cell maintenance.

2018-05-22 | GSE108181 | GEO

Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Spermatogonia PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [P6 iCLIP]

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Dazl HITS-CLIP on 25dpp C57BL/6 mouse testes

Project description:The autosomal gene Dazl is a member of highly conservative DAZ family, all of which contains a RNA binding domain and expresses in germ cells. In mice, Dazl plays an essential role in germ cell development, germ cell lost in Dazl knockout mouse on pure C57BL/6 strain background. Here we use HITS-CLIP to globally characterize the genome-wide target RNA of Dazl in mouse testes. Sequencing data provides large quantities of clues to help us to get close to the truth of Dazl function. We find over 1700 RNAs are bind by Dazl mainly at 3’UTR and the genes associated with transcription, RNA splicing and reproductive cellular process are enriched significantly.

2019-10-09 | GSE113710 | GEO

Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult PolyA-Seq]

Project description:Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult PolyA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Spermatogonia PolyA-Seq]

Project description:Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Spermatogonia PolyA-Seq]

| PRJNA429366 | ENA

Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [Adult RNA-Seq]

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Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions

Project description:Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions

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