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ABSTRACT: Metagenome of commercial bee pollen from Mexico

PROVIDER: PRJNA433224 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR6677927_1.fastq.gz	Fastqsanger.gz
	SRR6677927_2.fastq.gz	Fastqsanger.gz
	SRR7080747_1.fastq.gz	Fastqsanger.gz
	SRR7080747_2.fastq.gz	Fastqsanger.gz
	SRR7725250_1.fastq.gz	Fastqsanger.gz

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Project description:Commercial bee pollen from Chile

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A Combined Proteomic and Metabolomic Strategy for Allergens Characterization in Natural and Fermented Brassica napus Bee Pollen

Project description:Bee pollen is consumed for its nutritional and pharmacological benefits, but it also contains hazardous allergens which has not been identified. Here, we identified 2 potential allergens, glutaredoxin and oleosin-B2, in Brassica napus (B. napus) bee pollen using mass spectrometry-based proteomics analyses, and used bioinformatics to predict their antigenic epitopes. Comparison of fermented (by Saccharomyces cerevisiae) and unfermented bee pollen samples indicated that glutaredoxin and oleosin-B2 contents were significantly decreased following fermentation, while the contents of their major constituent oligopeptides and amino acids were significantly increased based on metabolomics analyses. Immunoblot analysis indicated that the IgE-binding affinity with extracted bee pollen proteins was also significantly decreased after fermentation, suggesting a reduction in the allergenicity of fermented bee pollen. Furthermore, fermentation apparently promoted the biosynthesis of L-valine, L-isoleucine, L-tryptophan and L-phenylalanine, as well as their precursors or intermediates. Thus, fermentation could potentially alleviate allergenicity, while also positively affecting nutritional properties of B. napus bee pollen. Our findings might provide a scientific foundation for improving the safety of bee pollen products to facilitate its wider application.

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Project description:Metagenome in commercial pollen from Europe

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Raw data from "Biotechnological approach for development and characterization of protein feed for Melipona quadrifasciata"

Project description:Analysis and comparison of the metabolomic profile of fermented pollen (colected by Melipona quadrifasciata stingless bee), fermented feed (mixture of fermented pollen with bran to feed Melipona quadrifasciata stingless bee) and bran (used in the mixture with fermented pollen). The abbreviations that name the raw files are: T7 - Fermented feed. POLEN - Fermented pollen. CTRL - Bran. FALSA - False organic solvent used (HPLC grade MeOH) and equipment system (UHPLC-HRMS).

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Nutrigenomics in honey bees: the impact of pollen nutrients on bee health

Project description:We studied the molecular mechanisms underlying the impact of pollen nutrients on honey bee (Apis mellifera) health and how those nutrients improve resistance to parasites. Using digital gene expression, we determined the changes in gene expression induced by pollen intake in worker bees parasitized or not by the mites Varroa destructor, known for suppressing immunity and decreasing lifespan of bees.

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Nutrigenomics in honey bees: the impact of pollen nutrients on bee health

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Protein profiling of human serum against a panel of allergens.

Project description:Current diagnostics for allergies, such as skin prick and radioallergosorbent tests, do not allow for inexpensive, high-throughput screening of patients. Additionally, extracts used in these methods are made from washed pollen that lacks pollen surface materials that may contain allergens. We sought to develop a high-throughput assay to rapidly measure allergen-specific IgE levels in sera and to explore the relative allergenicity of different pollen fractions (i.e. surface, cytoplasmic, commercial extracts). To do this, we generated a protein microarray containing surface, cytoplasmic, and commercial extracts from 22 pollen species, commercial extracts from nine non-pollen allergens, and five recombinant allergenic proteins. Arrays were incubated with <25uL of serum from 176 individuals and bound IgE was detected by indirect immunofluorescence, providing a high-throughput measurement of IgE levels.

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Project description:Background: Honey bee is a major insect used for pollination of a number of commercial crops worldwide. However, the number of managed honey bee colonies has recently declined in several countries, and a number of possible causes are proposed. Although the use of honey bees for pollination can be considered as disruption of the habitat, its effects on honey bees' physiology have never been addressed. In Japan, more than 100 thousands colonies are annually used for pollination, and intriguingly 80% of them are used in greenhouses. Recently, honey bee colonies have often collapsed when they are introduced into greenhouses. Thus, to suppress colony collapses and maintain the number of worker bees in the colonies are essential for successful long-term pollination in greenhouses and recycling honey bee colonies.

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Project description:To identify the potential proteins from bee pollen of Brassica by proteomics approach

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