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Analysis of gene expression in co-culture of PBMCs with ovarian cancer cell lines by High-Throughput Sequencing

ABSTRACT: Analysis of gene expression in co-culture of PBMCs with ovarian cancer cell lines by High-Throughput Sequencing

PROVIDER: PRJNA434447 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6743590_1.fastq.gz	Fastqsanger.gz
	SRR6743590_2.fastq.gz	Fastqsanger.gz
	SRR6743591_1.fastq.gz	Fastqsanger.gz
	SRR6743591_2.fastq.gz	Fastqsanger.gz
	SRR6743592_1.fastq.gz	Fastqsanger.gz

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Analysis of gene expression in co-culture of PBMCs with ovarian cancer cell lines by High-Throughput Sequencing

Project description:(Objectives) The goal of this study is to investigate paracrine effect of peripheral blood mononuclear cells (PBMCs) on ovarian cancer cell lines. (Methods) Transcription profiles of PBMC (control) and PBMC co-cultured with ovarian cancer cell lines (treatment) were generated by deep sequencing, in triplicate, using Illumina HiSeq X Ten. (Results and Conclusions) Transcriptome analysis of PBMCs has revealed the expression change when co-cultured with EOC cell lines with significantly in up-regulated genes; CDKN1B, GIMAP8 and SNN. The qRT-PCR validation have indicated that GIMAP8 was high expressed in EOC patients when compared with healthy female. We summarized that PBMCs were changed their expression as a result of paracrine signals from ovarian cancer cells.

2020-12-31 | GSE110745 | GEO

Analysis of gene expression in co-culture of PBMCs with lung cancer cell lines by High-Throughput Sequencing

Project description:Objective: The goal of this study is to investigate paracrine effect of Peripheral Blood Mononuclear Cells (PBMCs) on lung cancer cell lines. Method: Transcription profiles of PBMCs (control) and PBMCs co-cultured with lung cancer cell lines (treatment) were generated by deep sequencing,in triplicate, using Illumina HiSeq 4000. Results and Conclusions: With demonstrating of RNA sequencing approach, PBMCs has been shown the gene expression changed when co-cultured with lung cancer cell lines. The result revealed that up-regulated genes were 8,073 and 8,070 genes co-cultured with A549 and H292, respectively. In the other hand, down-regulated genes were 8,341 and 8,342 genes co-cultured with A549 and H292, respectively. Comparing the data to expression array databases from lung cancer patients, up-regulated genes were shown 29 genes co-cultured with A549 and 37 genes with H292 which, among them, there were 2 genes, ANKRD37 and BUB3, found in both treatments. Down-regulated genes were found 24 and 35 genes in the co-culture of A549 an d H292, respectively, that no similar genes found in both treatments.

2021-12-31 | GSE112005 | GEO

Analysis of gene expression in co-culture of PBMCs with Hepatocellular carcinoma by High-Throughput Sequencing

Project description:Objectives: To characterize the transcripts profile of PBMCs when co-cultured with HCC cell lines using High-Throughput Sequencing and To validate the differentially expressed genes via quantitative reverse transcription polymerase chain reaction (qRT-PCR) in PBMCs of HCC pateints. Methods: Transcription profiles of PBMC (control) and PBMC co-cultured with liver cancer cell lines (treatment) were generated by deep sequencing, in triplicate, using Illumina Illumina HiSeq 4000. Results and conclusion: Transcriptome analysis of PBMCs has revealed the presence of biological process alterations when co-cultured with HCC cell lines and qRT-PCR validation have indicated that four genes were up-reglurated in HCC patients when compared with healthy donors including IL1b, INHBA, PLOD2, PRG4.

2021-01-05 | GSE108796 | GEO

Analysis of gene expression in co-culture of PBMCs with breast cancer cell lines by High-Throughput Sequencing

Project description:The goal of this study is to investigate paracrine effect of peripheral blood mononuclear cells (PBMCs) on breast cancer cell lines. Transcription profiles of PBMCs (control) and PBMCs co-cultured with breast cancer cell lines (treatment) were generated by deep sequencing, in triplicate for MCF7 and duplicate for T47D, using Illumina HiSeq 4000. Transcriptome analysis of PBMCs has shown the gene expression changed when co-cultured with breast cancer cell lines. The result revealed significantly up- and down-regulated genes.

2021-12-31 | GSE112280 | GEO

Analysis of gene expression in co-culture of PBMCs with colon cancer cell lines by High-Throughput Sequencing

Project description:(Objectives) The goal of this study is to investigate paracrine effect of peripheral blood mononuclear cells (PBMCs) on colon cancer cell lines. (Method) Transcription profiles of PBMCs (control) and PBMCs co-cultured with colon cancer cell lines (treatment) were generated by deep sequencing, in triplicate for SW480 and duplicate for HT29, using Illumina HiSeq 4000. (Results and conclusion) Transcriptome analysis of PBMCs has shown the gene expression changed when co-cultured with colon cancer cell lines. The result revealed significantly up- and down-regulated genes.

2020-12-31 | GSE110989 | GEO

Analysis of gene expression in co-culture of PBMCs with breast cancer cell lines by High-Throughput Sequencing

Project description:Analysis of gene expression in co-culture of PBMCs with breast cancer cell lines by High-Throughput Sequencing

| PRJNA445463 | ENA

Analysis of gene expression in co-culture of PBMCs with lung cancer cell lines by High-Throughput Sequencing

Project description:Analysis of gene expression in co-culture of PBMCs with lung cancer cell lines by High-Throughput Sequencing

| PRJNA438933 | ENA

Analysis of gene expression in co-culture of PBMCs with colon cancer cell lines by High-Throughput Sequencing

Project description:Analysis of gene expression in co-culture of PBMCs with colon cancer cell lines by High-Throughput Sequencing

| PRJNA435504 | ENA

Analysis of gene expression in co-culture of PBMCs with Hepatocellular carcinoma by High-Throughput Sequencing

Project description:Analysis of gene expression in co-culture of PBMCs with Hepatocellular carcinoma by High-Throughput Sequencing

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Immune challenged SC-islets first response profiled by single cell transcription analysis after co-culture with human allogeneic PBMCs [scRNA-seq_invitro]

Project description:We performed an in vitro co-culture of allogeneic PBMCs and SC-islet clusters. SC-islet clusters were enriched for β cells (using CD49A magnetic sorting; SC-α still remain at lower numbers) (Veres et al., 2019), dissociated and reaggregated to obtain a more uniform cell count between wells. SC-islets were co-cultured with human allogeneic PBMCs for 24 or 48 hours. As controls [time(t)=0], SC-islets remained in culture without PBMC addition. These samples, in addition to PBMCs alone (t=0), were used for scRNA-seq (Figure 2A).

2022-09-01 | GSE200084 | GEO

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