Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global transcriptome analysis of HAP1 cells

ABSTRACT: Global transcriptome analysis of HAP1 cells

PROVIDER: PRJNA436386 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6793180_1.fastq.gz	Fastqsanger.gz
	SRR6793180_2.fastq.gz	Fastqsanger.gz
	SRR6793181_1.fastq.gz	Fastqsanger.gz
	SRR6793181_2.fastq.gz	Fastqsanger.gz
	SRR6793182_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Global transcriptome analysis of HAP1 cells

Project description:We analyzed the global change in transcription upon CHD3-KO and SENP1-KO compared to control HAP1 cells.

2018-08-05 | GSE111272 | GEO

Transcriptome regulated by NEIL2 in human HAP1 cells

Project description:To elucidate the role of DNA glycosylase NEIL2 in transcriptome regulation, we performed RNA sequencing in human HAP1 cells of Neil2 knockout and wild type. The tanscription count was extracted and the differential expressed genes (DEGs) were analysed.

2023-12-13 | GSE240564 | GEO

ATAC sequencing of HAP1 cells

Project description:We analysed the global effect of CHD3-KO and SENP1-KO HAP1 cell lines compared to control cell line, on chromatin accessibility using ATAC-seq.

2018-08-05 | GSE111047 | GEO

Gene expression analysis of human haploid cells (HAP1) depleted of SMARCB1 and SMARCA4

Project description:The aim of this study is to analyze the change in genome wide expression levels in HAP1 cells upon loss of SMARCB1, SMARCA4 or both these genes together. The SMARCB1 and SMARCA4 genes were the hits from a genome wide screen involving genetrap mutagenesis to find new players that are involved in sensitivity to Doxorubicin (Dox). It was found that loss of SMARCB1 and SMARCA4 genes impart resistance in HAP1 cells to Dox. To validate this, the genes were knocked out in HAP1 cells with CRISPR-Cas9 technology. Gene expression levels in SMARCB1 null, SMARCA4 null and SMARCB1-SMARCA4 double null cells were compared to wildtype HAP1 cells using RNAseq. From these experiments it was found that SMARCB1 loss caused several fold increase in ABCB1 gene levels. ABCB1 is an efflux pump in cells responsible for flushing out many small-molecule drugs. Further analysis of this gene confirmed that ABCB1 was the main factor responsible for Dox resistance upon SMARCB1 loss.

2016-08-22 | GSE75515 | GEO

Gene expression analysis of human haploid cells (HAP1) depleted of SMARCB1 and SMARCA4

2016-08-22 | E-GEOD-75515 | biostudies-arrayexpress

LFQ shotgun proteomic analysis of HAP1 NatC KO cells

Project description:The impact of NatC subunits NAA30, NAA35 and NAA38 on the human proteome (protein abundance). Analysis of HAP1 WT, NAA30 KO, NAA35 KO and NAA38 KO cells

2023-09-20 | PXD034104 | Pride

Transcriptome regulated by NEIL2 in human HAP1 cells

Project description:Transcriptome regulated by NEIL2 in human HAP1 cells

| PRJNA1004242 | ENA

RNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells

Project description:We performed a genome-scale screen for suppressors of interferon stimulated gene (ISG) expression in human haploid cells (HAP1). Ubiquitin specific peptidase 14 (USP14) was a significant hit. In order to validate USP14 as a regulator of ISG expression, we created knockouts of USP14 in HAP1 cells using CRISPR-Cas9 and performed RNA-seq on coding RNA from USP14 KO and WT cells. This data was used to determine if ISGs were upregulated in USP14 KO HAP1 cells.

2017-07-25 | E-MTAB-5865 | biostudies-arrayexpress

RNA-seq of coding RNA from DDX6 knockout human haploid (HAP1) cells

Project description:We performed a genome-scale screen for suppressors of interferon stimulated gene (ISG) expression in human haploid cells (HAP1). DEAD-box helicase 6 (DDX6) was a significant hit. In order to validate DDX6 as a regulator of ISG expression, we created knockouts of DDX6 in HAP1 cells using CRISPR-Cas9 and performed RNA-seq on coding RNA from DDX6 KO and WT cells. This data was used to determine if ISGs were upregulated in DDX6 KO HAP1 cells.

2017-07-25 | E-MTAB-5867 | biostudies-arrayexpress

Cell Surface Glycoengineering - HAP1 Cells

Project description:Cell Surface Glycoengineering of HAP1 Cells using CMP-Neu5Ac Derivatives

2022-06-26 | PXD024251 | Pride