Project description:Plant-based diets could be a key source of microRNAs in animals. Plant microRNAs are cross-kingdom gene expression regulators that could modulate mammalian gene expression, influencing their physiology. Therefore, it is important to identify the microRNA expression profile of plant foods in order to identify potential target genes and biological functions in the mammalian host. Next-generation sequencing was applied to identify microRNAs in RNA samples derived from nuts (walnut and almond), vegetables (spinach) and fruits (orange, apple, olive, pear, and tomato). Our data revealed that edible plant contain a large number and diverse type of microRNAs.
Project description:Gray mold disease affects fruits, vegetables and ornemental plants around the world, causing considerable losses every year. Its causing agent, the fungus Botrytis cinerea, produces infection cushions (IC) that are complex appressorial structure dedicated to the penetration of the plant tissues. A microarray analysis identified 1,231 up-regulated genes in IC-enriched mycelium, among which several genes coding for putative secreted proteins. These data were supported by qPCR analysis in vitro and in planta. They highlight a secretion of proteins involved in virulence like proteases, plant cell wall degrading enzymes and necrosis inducers. To validate these transcriptomic data, a proteomic analysis of the IC exo-proteome is required.
Project description:The molecular mechanisms by which dietary fruits and vegetables confer cardiometabolic benefits remain poorly understood. Historically, these beneficial properties have been attributed to the antioxidant activity of flavonoids. Here, we reveal that the host metabolic benefits associated with flavonoid consumption actually hinge on gut microbial metabolism. However, flavonoids are consumed in a largely glycosylated form, rendering them poorly available for small intestinal absorption and subjecting them to microbial metabolism in the colon. We show that a single gut microbial flavonoid catabolite is sufficient to reduce diet-induced cardiometabolic disease burden in mice. Dietary supplementation with elderberry extract attenuated obesity and continuous delivery of the catabolite 4-hydroxphenylacetic acid was sufficient to reverse hepatic steatosis. Analysis of human gut metagenomes revealed that under one percent contains a flavonol catabolic pathway, underscoring the rarity of this process. Our study will impact the design of dietary and probiotic interventions to complement traditional cardiometabolic treatment strategies.
Project description:Identification of protease targets is essential to understand the molecular mechanism by which a protease regulates the biological processes. In this study, we applied an iTRAQ-based quantitative proteomic approach to identify the potential targets of a vacuolar processing enzyme in the cellular context in tomato. Proteins were isolated from fruits of wild-type and RNAi tomato plants. More than 300 proteins were finally screened as significantly altered in the RNAi fruits. Based on Blast2go, a web-based bioinformatic tool that categorizes proteins according to their Gene Ontology (GO) annotations, the proteins experienced significant changes in abundance in the RNAi fruits were classified into fifteen functional categories.
2017-02-20 | PXD002980 | Pride
Project description:Potential probiotic candidate isolated from healthy buffalo uterus
