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RNA-seq data for tumor tissues from mice fed with normal diet (ND) or high cholesterol diet (HCD) II

ABSTRACT: RNA-seq data for tumor tissues from mice fed with normal diet (ND) or high cholesterol diet (HCD) II

PROVIDER: PRJNA473144 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7219360_1.fastq.gz	Fastqsanger.gz
	SRR7219360_2.fastq.gz	Fastqsanger.gz
	SRR7219361_1.fastq.gz	Fastqsanger.gz
	SRR7219361_2.fastq.gz	Fastqsanger.gz
	SRR7219354_1.fastq.gz	Fastqsanger.gz

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RNA-seq data for tumor tissues from mice fed with normal diet (ND) or high cholesterol diet (HCD)

Project description:To address how serum cholesterol affected tumor progression, C57BL/6J mice were placed on either normal diet or high cholesterol diet for 4 weeks, and then B16F10 cells were subcutaneously injected into the right flanks of mice. We performed transcriptome sequencing analysis (RNA-seq) on tumor tissues from ND and HCD groups.

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RNA-seq data for tumor tissues from mice fed with normal diet (ND) or high cholesterol diet (HCD) II

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RNA-seq data for tumor tissues from mice fed with normal diet (ND) or high cholesterol diet (HCD)

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Project description:Liver samples of mice harboring liver-specific deletion of Lats2 (Lats2-CKO) were compared to WT mice. This effect was tested along with the effect of diet - high cholesterol diet (Paigen-PD) vs. normal chow (ND). Expression array analysis on Affymetrix microarrays was carried out for livers from WT and Lats2-CKO mice fed ND or HCD for 9 weeks.

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Project description:Purpose: To investigate the effects of paternal high cholesterol diet on the F1 intimal transcriptome related to atherosclerosis. Methods:Three-week-old male LDL Receptor-deficient (LDLR-/-) male mice were fed a low-cholesterol diet (LCD) or high-cholesterol diet (HCD) for 8 weeks and then mated with age-matched control LDLR-/- female mice to generate F1 offspring. F1 LDLR-/- mice were fed a LCD for 16 weeks. Intimal RNA was extracted by injecting Trizol into the aorta and collecting the flow through for further processing. Results: . Total RNA isolated from the F1 intima were then subjected for RNA-sequencing analysis and results show that paternal hypercholesterolemia led to upregulation of 147 genes in the intimal of F1 female mice, many of which are involved in immune and inflammatory pathways.

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