Project description:We demonstrate a different strategy that successfully transformed an existing, functionally dispensable organ to regenerate another, functionally vital one in the body. Specifically, we injected STH into the mouse spleen to remodel its tissue structure, and implanted in there human or rat liver cell lines. The remodeled spleen developed an immunosuppressive, yet highly vascularized microenvironment, in which the trans-species cells proliferated fast and developed the functions of the human liver through 8 weeks, without exerting adverse responses.
Project description:We restored liver function in the spleen by direct reprogramming splenic fibroblasts in situ and thereby transformed the spleen into a “liver” with sufficient physiological functions within a living animal.
Project description:We restored liver function in the spleen by direct reprogramming splenic fibroblasts in situ and thereby transformed the spleen into a “liver” with sufficient physiological functions within a living animal.
Project description:We restored liver function in the spleen by direct reprogramming splenic fibroblasts in situ and thereby transformed the spleen into a “liver” with sufficient physiological functions within a living animal.
Project description:total RNA from mouse (male c57BL/6) spleen labeled with Cy3 vs total RNA from mouse (male c57BL/6) B cells treated with TGF-beta (transforming growth factor-beta) labeled with Cy5- time course with repeats Keywords: ordered