Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Paenibacillus phage PG1

ABSTRACT: Paenibacillus phage PG1 genome sequencing project

PROVIDER: PRJNA47551 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR100641.fastq.gz	Fastqsanger.gz
	GCA_000908775.1_sequence_report.txt	Txt

Items per page:

1 - 2 of 2

Similar Datasets

Burkholderia plantarii strain:PG1

Project description:Burkholderia plantarii strain:PG1 Genome sequencing

| PRJNA33837 | ENA

Lactiplantibacillus plantarum strain:PG1

Project description:Lactiplantibacillus plantarum strain:PG1 Genome sequencing and assembly

| PRJNA858574 | ENA

Rugosibacter aromaticivorans strain:PG1-Ca6

Project description:Rugosibacter aromaticivorans strain:PG1-Ca6 Genome sequencing

| PRJNA264992 | ENA

Escherichia coli strain:PG1 | isolate:PG1

Project description:Phage Genome sequencing

| PRJNA1084219 | ENA

Unlock the mystery of the hard-to-sequence phage genome: discovering a novel mechanism of bacterial immunity

Project description:Whole-genome sequencing is an important way to understand the genetic information, gene function, biological characteristics, and living mechanisms of organisms. There is no difficulty to have mega-level genomes sequenced at present. However, we encountered a hard-to-sequence genome of Pseudomonas aeruginosa phage PaP1. The shotgun sequencing method failed to dissect this genome. After insisting for 10 years and going over 3 generations of sequencing techniques, we successfully dissected the PaP1 genome with 91,715 bp in length. Single-molecule sequencing revealed that this genome contains lots of modified bases, including 51 N6-methyladenines (m6A) and 152 N4-methylcytosines (m4C). At the same time, further investigations revealed a novel immune mechanism of bacteria, by which the host bacteria can recognize and repel the modified bases containing inserts in large scale, and this led to the failure of the shotgun method in PaP1 genome sequencing. Strategy of resolving this problem is use of non-library dependent sequencing techniques or use of the nfi- mutant of E. coli DH5M-NM-1 as the host bacteria to construct the shotgun library. In conclusion, we unlock the mystery of phage PaP1 genome hard to be sequenced, and discover a new mechanism of bacterial immunity in present study. Methylation profiling of Pseudomonas aeruginosa phage PaP1 using kinetic data generated by single-molecule, real-time (SMRT) sequencing on the PacBio RS.

2013-08-22 | E-GEOD-50100 | biostudies-arrayexpress

The effect of kanamycin on Paenibacillus vortex

2012-01-24 | GSE35271 | GEO

Paenibacillus

Project description:Genome sequencing project of Paenibacillus spp.

| PRJDB6975 | ENA

Transcriptome analysis reveals that sporulation is used as an iron deficiency resistance mechanism in Paenibacillus riograndensis SBR5

Project description:Transcriptome analysis of Paenibacillus riograndensis SBR5.

2015-04-11 | GSE66816 | GEO

Burkholderia plantarii strain:PG1

Project description:Burkholderia plantarii strain:PG1 Raw sequence reads

| PRJNA925312 | ENA

The effect of kanamycin on Paenibacillus vortex

Project description:Transcriptional profiling of the bacteria Paenibacillus vortex comparing control untreated cells with kanamycin treated cells after 18 hours of exposure. Goal was to determine the effect of the antibiotic kanamycin in concentration which affect the colony morphology on global bacteria gene expression. Two-condition experiment, control cells vs. kanamycin treated cells. Biological replicates: 2 control replicates, 2 treated replicates. Pooling of 5 technical replicates for each biological replicate.

2012-01-24 | E-GEOD-35271 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data