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ABSTRACT: RNA sequencing of MC38 mouse tumor tissue with escalating drug dose

PROVIDER: PRJNA477829 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7421800_1.fastq.gz	Fastqsanger.gz
	SRR7421800_2.fastq.gz	Fastqsanger.gz
	SRR7421810_1.fastq.gz	Fastqsanger.gz
	SRR7421810_2.fastq.gz	Fastqsanger.gz
	SRR7421820_1.fastq.gz	Fastqsanger.gz

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Project description:RNA sequencing of MC38 mouse tumor tissue treated +/- drug and +/- immune cell depletion

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Non-homogenous intratumor ionizing radiation doses synergize with PD1 and CXCR2 blockade

Project description:Partial tumor irradiation combining low dose radiation with high dose radiation within the same tumor mass would prompt an antitumor effect when combined with immunomodulators. Partial irradiations (50%/50%) of subcutaneous MC38 tumors with millimetric precision to deliver low dose radiation (2Gy) and high dose radiation (16Gy) within the same tumor was performed and single-cell RNA sequencing of CD45+ cells analysis was done in both the two part of the tumor to identify tumor-infiltrating immune cells.

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Single cell TCR-seq profiling of T cells isolated from LNs of MC38 tumor-bearing mice

Project description:We investigate the single-cell landscape of the inflammatory mouse tumor model MC38, a C57BL/6 tumor cell line derived from colon adenocarcinoma. MC38 (diluted in HBSS and matrigel) was inoculated in the right unilateral flank (in the border of positions B2 and B3) of C57BL/6 mice (ref Study 16-3384 AV). Draining lymph nodes were taken one day after group-out (average 150-250 mm3 tumor size at day 0), approximately 14-19 days. Draining lymph nodes were dissociated and flow sorted accordingly to obtain the cells for 10x Chromium 5' Gene Expression Profiling and TCR sequencing.

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