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Transcriptome analysis of OGT-sufficient and OGT-deficient regulatory T (Treg) cells

ABSTRACT: Transcriptome analysis of OGT-sufficient and OGT-deficient regulatory T (Treg) cells

PROVIDER: PRJNA480070 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7495283.fastq.gz	Fastqsanger.gz
	SRR7495283_1.fastq.gz	Fastqsanger.gz
	SRR7495283_2.fastq.gz	Fastqsanger.gz
	SRR7495284.fastq.gz	Fastqsanger.gz
	SRR7495284_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of OGT-sufficient and OGT-deficient regulatory T (Treg) cells

Project description:To gain comprehensive insight into the OGT-dependent transcriptional program in Treg cells, we performed RNA-sequencing of isolated YFP+ Treg cells from Foxp3YFP-Cre/wtOgtwt/fl and healthy Foxp3YFP-Cre/wtOgtfl/fl females to avoid secondary changes in gene expression caused by inflammation. We were able to identify 269 differentially expressed genes including 154 downregulated and 115 upregulated with p values less than 0.01, OGT-deficient Treg cells had impaired suppressive function and attenuated IL2/STAT5 signaling pathway.

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RNA-seq analysis of transcriptomes of Noc4L deficient and the Nco4L sufficient Treg cells

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