Project description:Chronic stress triggers activation of the sympathetic nervous system (SNS) and drives malignancy. Using an immunodeficient murine system, we show that chronic stress promotes breast cancer stem-like properties via lactate dehydrogenase A (LDHA) dependent metabolic rewiring. In this dataset, we used microarray to characterize the mRNA expression profiles from primary PBS and Epinephrine -treated MDA-MB-231 tumor cells.
Project description:we investigated the effects of IH and epinephrine on gene expression in human aortic ECs using RNA-sequencing. We found a significant overlap between IH and epinephrine-induced differentially expressed genes (DEGs).
Project description:The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies. In the present investigation, we studied the proteome of biofilms and planktonic cultures of skin actinobacterium Cutibacterium acnes. In our investigation, we tested the effect of epinephrine in concentrations 5 nM (normal blood level) and 5 µM (1000-fold higher) on 72 h biofilms and planktonic cultures in comparison with controls without the hormone administration. Also, we compared the samples treated with epinephrine in different concentrations. Also, we investigated the differences between planktonic cultures and biofilms and how the hormone influences those differences. The present database contains the raw data of proteins changes in their concentrations in described conditions.
Project description:To investigate the trancriptomic changes of tumor endothelial cells (ECs) in response to STING activation, tumor ECs from MMTV-PyMT mouse model or implanted breast tumour model were either treated by PBS or cGAMP were sorted by FACS and single-cell RNA seq was performed.
Project description:The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies. In the present investigation, we studied the proteome of the biofilm matrix of the commensal actinobacterium Micrococcus luteus C01. In our investigation, we tested the effect of epinephrine in concentration 4.9×10-9 M on immature (24 h ) and mature (72 h) biofilms in comparison with controls without the hormone administration. We investigated the temporal changes in the matrix proteome during the incubation in control samples and in samples with epinephrine.
Project description:Breast cancer (BC) is the most prevalent and lethal tumor among women worldwide.
Although the antitumor effects of melatonin are well documented, its precise molecular
mechanisms in specific breast cancer subtypes remain unclear. This dataset provides
proteomic data from total protein extracts of two breast cancer cell lines [MCF-7
(ER+and PR+) and MDA-MB-468 (triple negative) treated with melatonin. The aim of
the study was to identify melatonin-induced changes in global protein expression and to
assess its potential as a therapeutic modulator in hormone-responsive and triple-negative
breast cancer.
Project description:Male and female mice were injected with NaCl vs epinephrine to induce acute paradoxical reversible heart failure akin to Takotsubo Syndrome with subsequent RNASeq analysis of left ventricular tissue.