Project description:Primary human peripheral blood mononuclear cells were isolated from full blood by standard ficoll centrifugation. Cells were washed and processed immediately.
Project description:Zoledronate is well known to act on osteoclasts to inhibit bone resorption.Zoledronate is administrated intravenously in clinical use, however, actions of zoledronate on cells in blood circulation are not fully understood. To understand its actions of human peripheral blood mononuclear cells (h-PBMC), we performed gene expression profiling in zoledronate-treated h-PBMC.
Project description:hole Genome Expression Profile of Human Peripheral Blood Mononuclear cells Exposed to Bacillus anthracis in vitro. Peripheral blood mononuclear cells exposed to a 1 MOI (multiplicity of infection pathogenic) of the B. anhracis spores.
Project description:Analysis of cryopreservation effects on peripheral blood mononuclear cells at gene expression level. The hypothesis tested in the present study was that cryopreservation has an influence on the transcriptome profile of peripheral blood mononuclear cells. Results indicated remarkable changes in expression patterns upon cryopreservation of PBMCs, with a strong loss of signal intensities to background levels for several transcripts. Total RNA obtained from isolated peripheral blood mononuclear cells of healthy human subjects eihter cryopreserved in liqiud nitrogen (frozen) or direclty lysed in Trizol after isolation (fresh).
Project description:hole Genome Expression Profile of Human Peripheral Blood Mononuclear cells Exposed to Bacillus anthracis in vitro. Peripheral blood mononuclear cells exposed to a 1 MOI (multiplicity of infection pathogenic) of the B. anhracis spores. Human Peripheral Blood Mononuclear cells Exposed to Bacillus anthracis in vitro