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Nicotinamide promotes cell survival and differentiation as kinase inhibitor in human pluripotent stem cells

ABSTRACT: Nicotinamide promotes cell survival and differentiation as kinase inhibitor in human pluripotent stem cells

PROVIDER: PRJNA496415 | ENA |

REPOSITORIES: ENA

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Nicotinamide promotes cell survival and differentiation as kinase inhibitor in human pluripotent stem cells

Project description:We examined the effect of nicotinamide on cell survival and differentiation in human pluripotent stem cells. Nicotinamide inhibited the phosphorylation of myosin light chain, suppressed actomyosin contraction, and led to improved cell survival after individualization. Then we analyzed the global gene expression profile after 24 hours of nicotinamide and ROCK inhibitor treatment, and found that the gene expression profile of human embryonic stem cell (hESC) treated with nicotinamide was much different from that of ROCK inhibitor treatment. Further analysis demonstrates that nicotinamide is an inhibitor of multiple kinases, including ROCK and CK1. We demonstrated that nicotinamide affected human embryonic stem cell (hESC) pluripotency and differentiation as a selective kinase inhibitor.

2018-10-29 | GSE121230 | GEO

Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes [2]

Project description:Nicotinamide, the amide form of vitamin B3, is essential to maintain the human fetal development. It benefits the ectoderm and endoderm development, but its influence in mesoderm differentiation is elusive. In this study, we reported that nicotinamide regulated the gene expression of cardiovascular system, and it induced functional cardiomyocytes which was independent of canonical WNT signaling. Through a kinase screening, we found that nicotinamide inhibited the activity of P38δ to promote cardiomyocyte differentiation. Furthermore, we compared the gene expression of cardiomyocytes induced by WNT inhibitor IWP-2, nicotinamide or P38 inhibitor SB202190. The results showed that the cardiomyocytes induced by nicotinamide had similar gene expression profile compared with P38 inhibitor, which indicated nicotinamide promoted cardiac differentiation via the inhibition of P38.

2021-12-01 | GSE154454 | GEO

Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes [1]

2021-12-01 | GSE154453 | GEO

High-Throughput Screening of Human Induced Pluripotent Stem Cell Cardiomyocytes Predicts Tyrosine Kinase Inhibitor Cardiotoxicity

Project description:High-Throughput Screening of Human Induced Pluripotent Stem Cell Cardiomyocytes Predicts Tyrosine Kinase Inhibitor Cardiotoxicity

2016-10-20 | GSE88944 | GEO

Tyrosine Kinase Inhibitor Cardiotoxicity

Project description:Tyrosine Kinase Inhibitor Cardiotoxicity

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Nicotinamide Promotes Cell Survival and Differentiation as Kinase Inhibitor in Human Pluripotent Stem Cells.

Project description:Nicotinamide, the amide form of vitamin B3, is widely used in disease treatments and stem cell applications. However, nicotinamide's impact often cannot be attributed to its nutritional functions. In a vitamin screen, we find that nicotinamide promotes cell survival and differentiation in human pluripotent stem cells. Nicotinamide inhibits the phosphorylation of myosin light chain, suppresses actomyosin contraction, and leads to improved cell survival after individualization. Further analysis demonstrates that nicotinamide is an inhibitor of multiple kinases, including ROCK and casein kinase 1. We demonstrate that nicotinamide affects human embryonic stem cell pluripotency and differentiation as a selective kinase inhibitor. The findings in this report may help researchers design better strategies to develop nicotinamide-related stem cell applications and disease treatments.

| S-EPMC6294242 | biostudies-literature

Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes

Project description:Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes

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Aged Mouse Muscle and Serum: Sedentary or Exercised Animals with or without Nicotinamide N-methyltransferase Inhibitor Treatment

Project description:Epigenomic profiling mouse gastrocnemius muscle and serum in aged female mice with/without exercise, with/without nicotinamide N-methyltransferase inhibitor treatment

2024-07-13 | GSE267903 | GEO

Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes [2]

Project description:Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes [2]

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Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes [1]

Project description:Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes [1]

| PRJNA646371 | ENA