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Human renal fibroblasts are strong immunomobilizers during a urinary tract infection mediated by uropathogenic Escherichia coli

ABSTRACT: Human renal fibroblasts are strong immunomobilizers during a urinary tract infection mediated by uropathogenic Escherichia coli

PROVIDER: PRJNA514304 | ENA |

REPOSITORIES: ENA

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Human renal fibroblasts are strong immunomobilizers during a urinary tract infection mediated by uropathogenic Escherichia coli

Project description:To prevent the onset of urosepsis and reduce mortality, a better understanding of how uropathogenic Escherichia coli (UPEC) manages to infiltrate the bloodstream through the kidneys is needed. The present study elucidates if human renal interstitial fibroblasts are part of the immune response limiting a UPEC infection, or if UPEC has the ability to modulate the fibroblasts for their own gain. Microarray results showed that upregulated genes were associated with an activated immune response. We also found that chemokines released from renal fibroblasts upon a UPEC infection could be mediated by LPS and triacylated lipoproteins activating the TLR2/1, TLR4, MAPK, NF-κB and PKC signaling pathways. Furthermore, UPEC was also shown to be able to adhere and invade renal fibroblasts, mediated by the P-fimbriae. Furthermore, it was found that renal fibroblasts were more immunoreactive than renal epithelial cells upon a UPEC infection. However, both renal fibroblasts and epithelial cells were equally efficient at inducing neutrophil migration. In conclusion, we have found that human renal fibroblasts can sense UPEC and mobilize a host response with neutrophil migration. This suggests that renal fibroblasts are not only structural cells that produce and regulate the extracellular matrix, but also highly immunoreactive cells.

2019-01-11 | GSE124917 | GEO

Genetically diverse uropathogenic Escherichia coli adopt a common transcriptional program in patients with urinary tract infections

Project description:Uropathogenic Escherichia coli (UPEC) is the major causative agent of uncomplicated urinary tract infections (UTIs). A common virulence genotype of UPEC strains responsible for UTIs is yet to be defined, due to the large variation of virulence factors observed in UPEC strains. We hypothesized that studying UPEC functional responses in patients might reveal universal UPEC features that enable pathogenesis. Here we identify a transcriptional program shared by genetically diverse UPEC strains isolated from 14 patients during uncomplicated UTIs. Strikingly, this in vivo gene expression program is marked by upregulation of translational machinery, providing a mechanism for the rapid growth within the host. Our analysis indicates that switching to a more specialized catabolism and scavenging lifestyle in the host allows for the increased translational output. Our study identifies a common transcriptional program underlying UTIs and illuminates the molecular underpinnings that likely facilitate the fast growth rate of UPEC in infected patients.

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Transcriptional responses of uropathogenic E. coli to stresses caused by sodium chloride or urea.

2012-04-05 | GSE28399 | GEO

Transcriptional responses of uropathogenic E. coli to stresses caused by sodium chloride or urea.

Project description:E. coli which cause urinary tract infections must respond to high osmolarity in the urinary tract as well as the presence of urea. We used microarrays to measure the differntial gene expression of uropathogenic strain CFT073 in conditions of high osmolarity of urea v. minimal media RNA was extracted from CFT073 in each growth condition and hybridized to an Affymetrix microarray. The differentially expressed genes were analyzed by expression pattern and function.

2012-04-04 | E-GEOD-28399 | biostudies-arrayexpress

Comparative Genomics of Urinary Tract Infectious Escherichia coli

Project description:Comparative genomic analysis of a range of urinary tract and urosepsis E.coli isolates

2011-05-20 | E-MEXP-3089 | biostudies-arrayexpress

human urinary tract metagenome

Project description:Urinary tract bacteria associated with urinary stone disease

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Using a Zebrafish Model to Identify Cellular Responses to Uropathogenic E. coli Bacteremia

Project description:Urinary tract infections (UTIs) are the second most common infections encountered in the pediatric population, second only to respiratory tract infections. UTIs are also a major cause of morbidity and mortality. UTIs can often ascend causing infection in the upper urinary tract or even progress to bacteremia or urosepsis. Urosepsis accounts for 10-30% of septic shock cases and Uropathogenic E.coli (UPEC) is responsible for almost 75% of cases. Therefore, increased understanding of the effects of urosepsis at the cellular and organ specific level will provide the foundation for improvements in clinical care.

2020-10-26 | GSE160038 | GEO

human urinary tract metagenome

Project description:human urinary tract metagenome Metagenome

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DNA Copy Number Variations in Children with Vesicoureteral Reflux and Urinary Tract Infections

Project description:RIVUR Trial participants had Agilent 1M probe and or Nimblegen 2.1M probe aCGH performed on genomic DNA. The study was designed to discover DNA copy number variations in genes critical in kidney/urinary tract development and urinary tract infection susceptibility. Reference DNA used is a single male sample

2019-04-15 | E-MTAB-7890 | biostudies-arrayexpress

RNA sequncing of HCN3+ urinary tract pacemaker cells

Project description:Purpose: to analyze the mRNA content of highly purified HCN3+ urinary tract pacemaker cells compared to HCN3- neighboring cells Methods: we sorted HCN3+ and HCN3- cells from E18.5 WT embryos and analyzed their content using RNA-seq Results: Identified differentially expressed transcripts in HCN3+ cells compared to HCN3- cells Conclusions: our study presents the first whole transcriptome analysis of HCN3+ urinary tract pacemaker cells that would provide a basis for the charactarization of the development and function of those cells

2018-04-17 | GSE103400 | GEO

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