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Unique and Shared Roles for Histone H3K36 Methylation States in Transcription Regulation Functions

ABSTRACT: Unique and Shared Roles for Histone H3K36 Methylation States in Transcription Regulation Functions

PROVIDER: PRJNA517160 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8489150.fastq.gz	Fastqsanger.gz
	SRR8489160.fastq.gz	Fastqsanger.gz
	SRR8489170.fastq.gz	Fastqsanger.gz
	SRR8489151.fastq.gz	Fastqsanger.gz
	SRR8489161.fastq.gz	Fastqsanger.gz

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Unique and Shared Roles for Histone H3K36 Methylation States in Transcription Regulation Functions

Project description:Set2 co-transcriptionally methylates lysine 36 of histone H3 (H3K36), producing mono-, di-, and trimethylation (H3K36me1/2/3). These modifications recruit or repel chromatin effector proteins important for transcriptional fidelity, mRNA splicing, and DNA repair. However, it was not known whether the different methylation states of H3K36 have distinct biological functions. Here, we use engineered forms of Set2 that produce different lysine methylation states to identify unique and shared functions for H3K36 modifications. Although H3K36me1/2 and H3K36me3 are functionally redundant in many SET2 deletion phenotypes, we found that H3K36me3 has a unique function related to Bur1 kinase activity and FACT (facilitates chromatin transcription) complex function. Further, during nutrient stress, either H3K36me1/2 or H3K36me3 represses high levels of histone acetylation and cryptic transcription that arises from within genes. Our findings uncover the potential for the regulation of diverse chromatin functions by different H3K36 methylation states.

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Set2-mediated H3K36 methylation states redundantly repress the production of antisense transcripts:: role in transcription regulation

Project description:Histone H3K36 can be added up to three methyl groups to form mono-, di-, and tri-methylation states. Recent study has shown that Set2 can suppress a specific group of antisense transcripts, which largely depends on the presence of H3K36 methylation. However, whether different methylation states possess distinct regulatory mechanisms on antisense transcripts is still unclear. In this study, we identified two yeast mutants that lack H3K36 di-methylation and tri-methylation, respectively. We also identified novel antisense transcripts in the absence of Set2 with own bioinformatics pipeline. Our study showed that the expression of these antisense transcripts does not affect the expression of corresponding sense genes. Different H3K36 methylation states (me2/me3) are not specific for the regulation of antisense expression, implying a co-regulation mechanism between them. Altogether, our study developed a method to identify new antisense transcripts, examined the potential effect of H3K36 di-methylation and tri-methylation on production of antisense transcripts. This study would shed light on the mechanism underlying how H3K36 methylation functions in the production of antisense transcripts.

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Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.

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