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Gene expression analysis of murine splenic IL-10+ and IL-10- B cells

ABSTRACT: Gene expression analysis of murine splenic IL-10+ and IL-10- B cells

PROVIDER: PRJNA521498 | ENA |

REPOSITORIES: ENA

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Gene expression analysis of murine splenic IL-10+ and IL-10- B cells

Project description:B cells belong to the adaptive immune system and have multiple functions. Besides their well characterized role in the promotion of the immune response, specific B cell subsets were shown to have an anti-inflammatory function. These cells are termed regulatory B cells and they exert their function mainly through the production of interleukin 10 (IL-10). This study focuses on B cells capable of producing IL-10 after 5 hours of stimulation with LPS, PMA and ionomycin, without any distinction based on surface markers.

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ATAC-seq examining chromatin accessibility of mouse splenic IL-10+ and IL-10-CD19+CD21hiCD24hi B cells

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VDJ analysis of IL-10+ and IL-10- splenic B1a, MZ, and FO B cells from sIgM-/- mouse

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IL-10 or IL-6 stimulation of IL-6-/- and IL-10-/- murine bone marrow derived macrophages in the presence of LPS

Project description:IL-10 or IL-6 stimulation of control 129xC57BL/6 murine bone marrow derived macrophages in the presence of LPS. We used microarrays to detail the global programme of gene expression changes in response to IL-6 or IL-10 stimulation in the presence of lipopolysaccharide. BMDMs were isolated from control, IL-6-/-, and IL-10-/- mice on a 129XBL/6 mixed background mice and differentiated in the presence of CSF-1 for 6-7 days. Cells were scraped and plated in 6 well plates at 2x10e6/well. Cells were washed with complete DMEM and rested for 1-2 hr before stimulation with combinations of IL-10 (10 ng/ml), IL-6 (2 ng/ml) or LPS (100 ng/ml) for 45 min or 180 mins. Complete biological replicates were performed. Keywords: time course

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Expression data from splenic IL-10 positive and IL-10 negative B cells of C57BL/6

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