Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification and quantification of modified nucleosides in Saccharomyces cerevisiae mRNAs

ABSTRACT: Identification and quantification of modified nucleosides in Saccharomyces cerevisiae mRNAs

PROVIDER: PRJNA521783 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8558675.fastq.gz	Fastqsanger.gz
	SRR8558676.fastq.gz	Fastqsanger.gz
	SRR8558677.fastq.gz	Fastqsanger.gz
	SRR8558678.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Identification and quantification of modified nucleosides in Saccharomyces cerevisiae mRNAs

Project description:Post-transcriptional modifications to messenger RNAs (mRNAs) have the potential to alter the biological function of this important class of biomolecules. The study of mRNA modifications is a rapidly emerging field, and the full complement of chemical modifications in mRNAs is not yet established. We sought to identify and quantify the modifications present in yeast mRNAs using an ultra-high performance liquid chromatography tandem mass spectrometry method to detect 40 nucleoside variations in parallel. We observe six modified nucleosides with high confidence in highly purified mRNA samples (N7-methylguanosine, N6-methyladenosine, 2’-O-methylguanosine, 2’-O-methylcytidine, N4-acetylcytidine and 5-formylcytidine), and identify the yeast protein responsible for N4-acetylcytidine incorporation in mRNAs, Rra1. Additionally, we find that mRNA modification levels change in response to heat shock, glucose starvation and/or oxidative stress. This work expands the repertoire of potential chemical modifications in mRNAs, and highlights the value of integrating mass spectrometry tools in the mRNA modification discovery and characterization pipeline.

2019-06-20 | GSE126405 | GEO

Identification of Mettl3-dependent m6A-modified mRNAs upon DNA-damage

Project description:The goal of the study was to identify those mRNAs that are m6A-modified in a Mettl3-dependent manner under DNA-damage condition in oncogene-expressing mouse embryonic fibroblasts

2022-07-07 | GSE169343 | GEO

Identification of Mettl3-dependent m6A-modified mRNAs upon DNA-damage

Project description:Identification of Mettl3-dependent m6A-modified mRNAs upon DNA-damage

| PRJNA716308 | ENA

Identification of NAD+ capped mRNAs in S. cerevisiae

Project description:We report the identification of NAD-RNA in the yeast Saccharomyces cerevisiae under two distinct growth mediums.

2016-11-30 | GSE90636 | GEO

Global m6A-modified mRNAs in the uterus of wild-type mouse

Project description:Global m6A-modified mRNAs in the uterus of wild-type mouse were analyzed by using methylated RNA immunoprecipitation sequencing (MeRIP-seq)

2022-11-18 | GSE211521 | GEO

Identification and Quantification of Modified Nucleosides in <i>Saccharomyces cerevisiae</i> mRNAs.

Project description:Post-transcriptional modifications to messenger RNAs (mRNAs) have the potential to alter the biological function of this important class of biomolecules. The study of mRNA modifications is a rapidly emerging field, and the full complement of chemical modifications in mRNAs is not yet established. We sought to identify and quantify the modifications present in yeast mRNAs using an ultra-high performance liquid chromatography tandem mass spectrometry method to detect 40 nucleoside variations in parallel. We observe six modified nucleosides with high confidence in highly purified mRNA samples (N7-methylguanosine, N6-methyladenosine, 2'-O-methylguanosine, 2'-O-methylcytidine, N4-acetylcytidine, and 5-formylcytidine) and identify the yeast protein responsible for N4-acetylcytidine incorporation in mRNAs (Rra1). In addition, we find that mRNA modification levels change in response to heat shock, glucose starvation, and/or oxidative stress. This work expands the repertoire of potential chemical modifications in mRNAs and highlights the value of integrating mass spectrometry tools in the mRNA modification discovery and characterization pipeline.

| S-EPMC7254066 | biostudies-literature

Global m6A-modified mRNAs in mouse uterus on gestational day 4 [meRIP-seq]

Project description:Global m6A-modified mRNAs in wild-type mouse uterus on gestational day 4 were analyzed by using methylated RNA immunoprecipitation sequencing (MeRIP-seq)

2024-04-12 | GSE263379 | GEO

Identification of the mRNAs interacting with the Nup59 nucleoporin using RNA-IP and microarrays in budding yeast

Project description:To specifically explore such mRNA localization events, we immunopurified the entire Nuclear Pore Complex scaffold using Nup59 as a bait and systematically analyzed all co-isolating mRNAs using Agilent microarrays in the budding yeast Saccharomyces cerevisiae. A mock IP was performed as a control experiment, using a yeast strain in which Nup59 was untagged. Two replicates of the Nup59 IP and of the mock IP were performed. The mRNAs with a significant enrichment (p<0.05 in the bayesian test of the LIMMA package) in the mock IP were removed from the further analyses of the Nup59-IP.

2021-03-31 | E-MTAB-9521 | biostudies-arrayexpress

Global m6A-modified mRNAs in the uterus of wild-type mouse

Project description:Global m6A-modified mRNAs in the uterus of wild-type mouse

| PRJNA870721 | ENA

Global m6A-modified mRNAs in undecidualized and decidualized primary human endometrial stromal cells [meRIP-seq]

Project description:Global m6A-modified mRNAs in undecidualized and decidualized primary human endometrial stromal cells were analyzed by using methylated RNA immunoprecipitation sequencing (MeRIP-seq)

2024-04-14 | GSE263331 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data