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Melan-a cells expressing reduced level of Dicer and control Melan-a cells

ABSTRACT: Melan-a cells expressing reduced level of Dicer and control Melan-a cells

PROVIDER: PRJNA528796 | ENA |

REPOSITORIES: ENA

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microRNA levels data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells

Project description:To investigate the molecular mechanism of the hypopigmentation observed in Dicer KO mice, Dicer knockdown was realised in vitro in normal C57BL/6 mouse melanocyte Melan-a cells. Transient transfection of Melan-a cells with siRNA directed against Dicer reduced Dicer protein levels to approximately 40% of that of siScr Melan-a cells 24 hours after transfection. We analyzed the miRnome of Melan-a cells 24 and 48 hours after transfection with siDicer or siScr to understand the molecular mechanisms involved in Dicer-dependent melanocyte migration.

2022-03-22 | GSE128754 | GEO

Gene expression data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells [48 hours]

Project description:To investigate the molecular mechanism of the hypopigmentation observed in Dicer KO mice, Dicer knockdown was realised in vitro in normal C57BL/6 mouse melanocyte Melan-a cells. Transient transfection of Melan-a cells with siRNA directed against Dicer reduced Dicer protein levels to approximately 40% of that of siScr Melan-a cells 48 hours after transfection. We analyzed the transcriptome of Melan-a cells 48 hours after transfection with siDicer or siScr to understand the molecular mechanisms involved in Dicer-dependent melanocyte migration.

2022-03-22 | GSE128756 | GEO

Gene expression data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells [24 hours]

Project description:To investigate the molecular mechanism of the hypopigmentation observed in Dicer KO mice, Dicer knockdown was realised in vitro in normal C57BL/6 mouse melanocyte Melan-a cells. Transient transfection of Melan-a cells with siRNA directed against Dicer reduced Dicer protein levels to approximately 40% of that of siScr Melan-a cells 24 hours after transfection. We analyzed the transcriptome of Melan-a cells 24 hours after transfection with siDicer or siScr to understand the molecular mechanisms involved in Dicer-dependent melanocyte migration.

2022-03-22 | GSE128755 | GEO

microRNA levels data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells

Project description:microRNA levels data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells

| PRJNA528797 | ENA

Gene expression data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells [24 hours]

Project description:Gene expression data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells [24 hours]

| PRJNA528798 | ENA

Gene expression data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells [48 hours]

Project description:Gene expression data from Melan-a cells expressing reduced level of Dicer and control Melan-a cells [48 hours]

| PRJNA528799 | ENA

Melan-a cells expressing reduced level of Dicer and control Melan-a cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-03-22 | GSE128757 | GEO

DICER-interacting partners in juvenile mouse testes

Project description:DICER has a well-characterized role in the processing of microRNAs (miRNAs) and small interfering RNAs (siRNA) that are important for post-transcriptional gene regulation. Emerging evidence suggests that DICER also has several non-canonical functions beyond miRNA/siRNA biogenesis, for example in transcriptional gene silencing at the chromatin level, as well as in RNA degradation and maintenance of genomic integrity. We have shown that the function of DICER in germ cells is essential for normal spermatogenesis; male mice lacking DICER in postnatal male germ cells are infertile due to severe defects in haploid differentiation. To better understand the function of DICER in male germ cells, we immunoprecipitated DICER from juvenile mouse testes and performed mass spectrometric analysis to identify DICER-interacting proteins.

2020-06-03 | PXD017772 | Pride

Definition of the Dicer-2 interactome during viral infection in Drosophila melanogaster

Project description:Although many in vitro studies have helped us understand how Dicer-2 is able to discriminate between different dsRNA substrate termini, much less is known about how this translates to the in vivo recognition of viral dsRNA. Indeed, Dicer-2 associates with several dsRNA-binding proteins (dsRBPs), which can modify its specificity for a substrate, however it remains unknown how Dicer-2 is able to recognize the protected termini of viral dsRNAs. Therefore, in order to study how the ribonucleoprotein network of Dicer-2 impacts antiviral immunity, we used an IP-MS approach to identify interactants of several fly lines expressing different versions of GFP:Dicer-2. By combining the global analysis of the Dicer-2 interactome with different line-specific analyses, we were able to both obtain a global overview of the partners of Dicer-2 in vivo, and study how this interactome was modulated by different factors such as the infection and/or the presence of different point mutations on the helicase or RNase III domains of GFP:Dicer-2. This allowed the identification of several new Dicer-2 interactants as well as new pro- and antiviral factors that had an impact on DCV infection. In addition, this work provides a resource composed of several candidates, available to the scientific community that can now be investigated further to gain a better understanding of the proteins involved in Dicer-2-mediated antiviral RNAi.

2025-04-15 | PXD038898 | Pride

Expression data from Dicer WT and Dicer KO mouse CD4 T cells.

Project description:miRNA regulate gene expression at the post-transcriptionnal level. To gain further insight into this process, we analysed by Affymetrix microarray, the transcriptome of Dicer WT or Dicer deleted mouse CD4 T cells.

2015-02-15 | GSE59848 | GEO

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