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Bisulfite sequencing of medaka PGCs methylomes

ABSTRACT: Bisulfite sequencing of medaka PGCs methylomes

PROVIDER: PRJNA533476 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8925590.fastq.gz	Fastqsanger.gz
	SRR8925591.fastq.gz	Fastqsanger.gz
	SRR8925592.fastq.gz	Fastqsanger.gz
	SRR8925593.fastq.gz	Fastqsanger.gz
	SRR8925584.fastq.gz	Fastqsanger.gz

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Bisulfite sequencing of medaka PGCs methylomes

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Project description:During development, the inherited DNA methylation patterns from the parental gametes needs to be remodeled into a state compatible with embryonic pluripotency. In Zebrafish, this remodeling is achieved by the maternal methylome becoming hypomethylated to match the paternal methylome. However, how this is achieved in medaka (another teleost fish) is currently not known. Moreover, how DNA methylation remodeling is impacted in hybrid organisms, and the effects this may have on their development, is also not known. Here we address these questions by generation whole genome bisulfite sequencing data for zebrafish, medaka and zebrafish medaka embryos.

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Effects of Potassium Perchlorate on Developing Medaka Primordial Germ Cells

Project description:The present study examined the effects of 0, 10, 100, and 1000 μg/L potassium perchlorate exposure on PGCs of medaka embryos and the expression pattern of genes that maintain PGCs integrity and epigenetic processes.

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Reduced representation bisulfite sequencing (RRBS) for primordial germ cells and somatic cells during zebrafish early embryogenesis

Project description:Zebrafish Primordial Germ Cells (PGCs) and somatic cells were isolated via FACS from Buc-GFP fish strain. Reduced representation bisulfite sequencing (RRBS) was performed on PGCs and somatic cells at high(6000-7000 cells per replicate), dome (7500 cells per replicate)and prim-5 stages(4500-5000 cells per replicate). Two replicates were collected for each cell type and stage.

2020-12-24 | E-MTAB-9858 | biostudies-arrayexpress

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