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Investigating Proteome and Transcriptome Defense Response of Grapes Induced by Yarrowia lipolytica

ABSTRACT: Investigating Proteome and Transcriptome Defense Response of Grapes Induced by Yarrowia lipolytica

PROVIDER: PRJNA534483 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8950070_1.fastq.gz	Fastqsanger.gz
	SRR8950070_2.fastq.gz	Fastqsanger.gz
	SRR8950069_1.fastq.gz	Fastqsanger.gz
	SRR8950069_2.fastq.gz	Fastqsanger.gz

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Investigating Proteome and Transcriptome Defense Response of Grapes Induced by Yarrowia lipolytica

Project description:We report the application of next generation sequencing technology for analyzing the differentially expressed genes of grapes after treated with or without Y. lipolytica. After analyzing the differentially expressed gene, we selected some important genes related to the resistance of grapes to do RT-qPCR.

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Absolute expression level of Yarrowia lipolytica genes

Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Yarrowia lipolytica genes Total RNA was collected in mid-log phase from Yarrowia lipolytica cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Yarrowia lipolytica.

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Transcriptome change in response to ochratoxin A (OTA) in Yarrowia lipolytica Y-2

Project description:The yeast Yarrowia lipolytica Y-2 shown the capability to degrade OTA by the intracellular enzymes. However, the enzymes which risponsible for the degradation process was unkonown. Transcriptome change in response to mycotoxin OTA was analyzed. The molecular mechanism of Yarrowia lipolytica Y-2 withstand OTA was revealed.

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