Project description:The acidic microenvironment plays a key role to result in the poor survival and limited function of transplanted stem cells in ischemic diseases.In this study, different pH levels of medium were performed to treat adipose-derived stem cells (ADSCs) with 24/48h time- exposure, to explore the effect of extracellular acidosis on ADSCs.
Project description:The goal of this study was to understand the effect of human adipose stem cell and its derived exosomes on mouse chronic liver fibrosis. After eight weeks of intraperitoneal injection of CCl4, we treated 200μl PBS, normal human adipose stem cell (1×106/200μl PBS), human adipose stem cell-derived exosomes(250μg/200μl PBS) once a week for 4 weeks. Then the livers were collected and isolated RNA for RNA-sequencing to explore alterations in mRNA expression between different treatments
Project description:This project is a report of protemoic data of secretome from human adipose tissue-derived stem cells. The ADSCs were cultured in serum-free condition, and LC-MS/MS identification was conducted to analyze the ingredients in the secretome.
Project description:Proteomics for sorted low vs. high 20% of lamin A/C-expressing primary, human, adipose-derived stem cells compared to an unsorted population
Project description:Adaptation to acidosis has been reported to induce a shift towards fatty acid metabolism. We observed that conditioned medium of HCT, FaDu and SiHa adapted to acidosis (pH6.5) induced higher lipolysis of primary subcutaneous and mesenteric adipocytes compared to cancer cells maintained at pH7.4. In order to identify the prolipolytic factors induced by acidosis, we examined the differences between cancer cells adapted to pH6.5 and those maintained at pH7.4 by RNA sequencing. Cancer cells adapted to pH6.5 showed profound alterations in their gene expression compared with cancer cells maintained at pH7.4. Here, we focused on predicted secreted genes. We determined that 84 altered genes predicted to be secreted proteins overlapped between the 3 cancer cell lines, 24 of which were overexpressed. The role of these overexpressed genes was determined in vitro. We studied their role in inducing adipocyte lipolysis and in the depletion of adipose tissue observed in cancer cachexia.
Project description:Proteome experiment was peformed on exosomes of human minor salivary gland mesenchymal stem cells and adipose-derived stem cells to find out the same points and difference of these two kinds of exosomes, which can hopefully give further guidance on further therapy research
Project description:we analyzed the expression level change of transcription factors in adipose derived stem cells during osteogenic differentiation and found a candidate target gene, Sox11. We defined that Sox11 suppresses osteogenic differentiation through overexpression and knock down of Sox11. total RNA obtained from adipose derived stem cells subjected to 1,3,6,10 or 14 days in osteogenic differentiation compared to undifferentiated control adipose derived stem cells.