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Next Generation Sequencing Facilitates Quantitative Analysis of Developed Seed Transcriptomes in Linseed Flax

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Developed Seed Transcriptomes in Linseed Flax

PROVIDER: PRJNA539945 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing Facilitates Quantitative Analysis of Developed Seed Transcriptomes in Linseed Flax

Project description:The goal of the study is to identify gene expression during seed development process in linseed flax.

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Flax cell wall proteome - A cell wall proteome and targeted cell wall analyses provide novel information on hemicellulose metabolism in flax

Project description:Proteomic analyses of four different flax organs/tissues (inner-stem, outer-stem, leaves and roots) enriched in proteins from 3 different sub-compartments (soluble-, membrane-, and cell wall-proteins) was combined with publically available data on flax seed and whole-stem proteins to generate a flax protein database containing 2996 non-redundant total proteins. Examination of the proteins present in different flax organs/tissues provided a detailed overview of cell wall metabolism and highlighted the importance of hemicellulose and pectin remodeling in stem tissues.

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RNAseq of WT + pctrl, ∆flaX + pctrl and ∆flaX + pPflaAX in V. cholerae

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flax

Project description:flax genome resequencing Raw sequence reads

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gene expression analysis in different tissues of Flax (Linum usitatissimum L.)

Project description:We developped a new oligo microarray platform to analyse flax transcriptome. Here, we validated this microarray on several tissues of flax, at different developmental stages.

2010-06-15 | GSE21868 | GEO

Alternative splicing and DNA methylation interact in linseed (Linum usitatissimum L.) response to different drought stresses

Project description:To investigate changes in genome methylation in flax seedlings under drought stress, we selected a drought-tolerant flax variety (Z141) and a drought-sensitive flax variety (NY-17) We then performed genome methylation analysis using data obtained from Z141 and NY-17 leaf tissue BS-seq at four different treatments (DS, RW, RD and CK).

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Effect of Supplementing Linseed in the Transition Period on Mammary Gland Tissue of dairy Cows

Project description:The aim of this study was to determine the effects of linseed dietary supplementation on gene expression in the mammary gland of grazing dairy cows. Milk composition and gene expression in the mammary gland tissue were evaluated in dairy cows supplemented with linseed. The linseed supplementation improves the health and nutrition quality aspects of dairy milk, but also affects the gene networks expression signature associated with cellular growth and proliferation, cell-death, signalling, nutrient metabolism, and immune response, and in turn, the mammary gland integrity and health.

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Project description:Flax rust effector transient expression in flax leaves RNA seq

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gene expression analysis in different tissues of Flax (Linum usitatissimum L.)

Project description:We developped a new oligo microarray platform to analyse flax transcriptome. Here, we validated this microarray on several tissues of flax, at different developmental stages. 30 chips study using total RNA from 9 different tissue samples of Flax (Leaf sample at green capsule stage, stem inner tissue at vegetavie stage, stem outter tissue at vegetative stage, stem inner tissue at green capsule stage, stem outter tissue at green capsule stage, roots, embryos of 10, 20 and 40 days after flowering) for an overall survey of microarry accuracy and total RNA from 6 stem tissue at green capsule stage (3 of the cultivar 'Belinka' and 3 of the cultivar 'Drakkar') for an analysis of biological replicates reproducibility.

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