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Transcriptomic profiling of zebrafish livers with nAtf6 overexpression at collected at 78 and 120 hpf

ABSTRACT: Transcriptomic profiling of zebrafish livers with nAtf6 overexpression at collected at 78 and 120 hpf

PROVIDER: PRJNA541476 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13912630_1.fastq.gz	Fastqsanger.gz
	SRR13912630_2.fastq.gz	Fastqsanger.gz
	SRR13912631_1.fastq.gz	Fastqsanger.gz
	SRR13912631_2.fastq.gz	Fastqsanger.gz
	SRR13912632_1.fastq.gz	Fastqsanger.gz

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Transcriptomic profiling of zebrafish livers with nAtf6 overexpression at collected at 78 and 120 hpf

Project description:Activting transcription factor 6 (Atf6) is one of three main mediators of the Unfolded Protein Response and it gets cleaved cleaved in the Golgi and its N-terminal consisting of the bZip domain translocates into the nucleus to generate a transcriptional response (nAtf6). This study carries out RNAseq on an established transgenic overexpression of nAtf6 in zebrafish hepatocytes ( tg(fabp10:nAtf6-cherry; cmlc2:GFP)-C allele). Samples were collected at 78 and 120 hpf to detect gene expression changes at early and late time points after nAtf6 is overexpressed.

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Project description:The goal of this study is genomic chromatin accessibility of 120 hpf zebrafish livers by ATAC-Seq. ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) and the DNA methylation profile using reduced representation bisulfite sequencing (RRBS).

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