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Rickettsia parkeri strain:Atlantic Rainforest

ABSTRACT: Rickettsia parkeri strain:Atlantic Rainforest Genome sequencing

PROVIDER: PRJNA542380 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR9899747_1.fastq.gz	Fastqsanger.gz
	SRR9899747_2.fastq.gz	Fastqsanger.gz
	SRR9899748_1.fastq.gz	Fastqsanger.gz

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Project description:We describe the clinical, serologic, and molecular findings of a new human rickettsiosis in Colombia. Antibodies against Rickettsia spp. were detected. PCR showed amplification of genes for R. parkeri strain Atlantic Rainforest. This new rickettsiosis of minor virulence could explain some of the undifferentiated acute febrile diseases in Colombia.

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Whole-Genome Sequence of Rickettsia parkeri Strain Atlantic Rainforest, Isolated from a Colombian Tick.

Project description:Rickettsia parkeri is classified as a member of the alphaproteobacterial microorganisms, genus Rickettsia Here, we report the complete genome sequence of Rickettsia parkeri strain Atlantic Rainforest, which was isolated from an Amblyomma ovale tick collected in the municipality of Necoclí, Colombia.

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Rickettsia parkeri strain Atlantic rainforest in ticks (Acari: Ixodidae) of wild birds in Arauca, Orinoquia region of Colombia.

Project description:Birds are important hosts for the development of the immature stages of several tick species that are vectors for disease-causing microorganisms in animals and humans. Colombia has the highest number of bird species worldwide; however, there is scarce data on the role of birds in the circulation of ticks and their associated pathogens, such as rickettsiae. The department of Arauca has a high diversity of resident and migratory (boreal and austral) birds and ticks associated with the transmission of Rickettsia. The objective of this research was to identify tick species parasitizing birds and to detect Rickettsia species in these ectoparasites. We conducted samplings in the municipalities of Arauca, Cravo Norte, and Tame between November of 2018 and August of 2019. Birds were captured using mist nets and examined for the presence of tick species. The collected ticks were morphologically and molecularly identified. Furthermore, we detected rickettsiae in ticks by amplifying fragments of the citrate synthase (gltA) and outer membrane protein (ompB) genes. We captured 606 birds belonging to 25 families and 115 species. Tick infestation rate was 3.3% (20/606) in the birds captured and eight new associations between wild birds and ticks are reported for the American continent. We identified four tick species: Amblyomma nodosum, Amblyomma longirostre, Amblyomma mixtum, and Amblyomma sp.. Moreover, we confirmed the presence of Rickettsia parkeri strain Atlantic rainforest in A. nodosum, a medically-relevant rickettsia due to cases of rickettsiosis in the American continent. This finding manifests the importance of wild birds as hosts and dispersal agents of ticks infected with pathogenic rickettsiae, as well as the need to monitor migratory birds in the Orinoquia and other regions of Colombia and America.

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Genomic comparison of virulent Rickettsia rickettsii Sheila Smith and avirulent Rickettsia rickettsii Iowa

Project description:Comparison of gene expression between the virulent Rickettsia rickettsii R strain and avirulent Rickettsia rickettsii Iowa. Keywords: virulent vs avirulent

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Genomic comparison of virulent Rickettsia rickettsii Sheila Smith and avirulent Rickettsia rickettsii Iowa

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A human case of spotted fever caused by Rickettsia parkeri strain Atlantic rainforest and its association to the tick Amblyomma ovale.

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