Project description:We describe the clinical, serologic, and molecular findings of a new human rickettsiosis in Colombia. Antibodies against Rickettsia spp. were detected. PCR showed amplification of genes for R. parkeri strain Atlantic Rainforest. This new rickettsiosis of minor virulence could explain some of the undifferentiated acute febrile diseases in Colombia.

| S-EPMC7706929 | biostudies-literature

Whole-Genome Sequence of Rickettsia parkeri Strain Atlantic Rainforest, Isolated from a Colombian Tick.

Project description:Rickettsia parkeri is classified as a member of the alphaproteobacterial microorganisms, genus Rickettsia Here, we report the complete genome sequence of Rickettsia parkeri strain Atlantic Rainforest, which was isolated from an Amblyomma ovale tick collected in the municipality of Necoclí, Colombia.

| S-EPMC6763642 | biostudies-literature

Rickettsia parkeri strain Atlantic rainforest in ticks (Acari: Ixodidae) of wild birds in Arauca, Orinoquia region of Colombia.

Project description:Birds are important hosts for the development of the immature stages of several tick species that are vectors for disease-causing microorganisms in animals and humans. Colombia has the highest number of bird species worldwide; however, there is scarce data on the role of birds in the circulation of ticks and their associated pathogens, such as rickettsiae. The department of Arauca has a high diversity of resident and migratory (boreal and austral) birds and ticks associated with the transmission of Rickettsia. The objective of this research was to identify tick species parasitizing birds and to detect Rickettsia species in these ectoparasites. We conducted samplings in the municipalities of Arauca, Cravo Norte, and Tame between November of 2018 and August of 2019. Birds were captured using mist nets and examined for the presence of tick species. The collected ticks were morphologically and molecularly identified. Furthermore, we detected rickettsiae in ticks by amplifying fragments of the citrate synthase (gltA) and outer membrane protein (ompB) genes. We captured 606 birds belonging to 25 families and 115 species. Tick infestation rate was 3.3% (20/606) in the birds captured and eight new associations between wild birds and ticks are reported for the American continent. We identified four tick species: Amblyomma nodosum, Amblyomma longirostre, Amblyomma mixtum, and Amblyomma sp.. Moreover, we confirmed the presence of Rickettsia parkeri strain Atlantic rainforest in A. nodosum, a medically-relevant rickettsia due to cases of rickettsiosis in the American continent. This finding manifests the importance of wild birds as hosts and dispersal agents of ticks infected with pathogenic rickettsiae, as well as the need to monitor migratory birds in the Orinoquia and other regions of Colombia and America.

| S-EPMC7502793 | biostudies-literature

Nanorana parkeri isolate:BGI_ZX_2015

Project description:Nanorana parkeri isolate:BGI_ZX_2015 Genome sequencing and assembly

| PRJNA243398 | ENA

The ankyrin repeat protein RARP-1 is a periplasmic factor that supports Rickettsia parkeri growth and host cell invasion

Project description:Rickettsia spp. are obligate intracellular bacterial pathogens that have evolved a variety of strategies to exploit their host cell niche. However, the bacterial factors that contribute to this intracellular lifestyle are poorly understood. Here, we show that the conserved ankyrin repeat protein RARP-1 supports Rickettsia parkeri infection. Specifically, RARP-1 promotes efficient host cell entry and growth within the host cytoplasm, but is not necessary for cell-to-cell spread or evasion of host autophagy. We further demonstrate that RARP-1 is not secreted into the host cytoplasm by R. parkeri. Instead, RARP-1 resides in the periplasm, and we identify several binding partners that are predicted to work in concert with RARP-1 during infection. Altogether, our data reveal that RARP-1 plays a critical role in the rickettsial life cycle.

2022-02-18 | MSV000088867 | MassIVE

RNA-seq reveals genes regulated by type I interferon during Rickettsia infection in WT and Irf-deficient bone marrow-derived macrophages

Project description:We report transcript abundance in murine bone marrow-derived macrophages (BMDMs) upon Rickettsia parkeri infection and treatment with type I interferon (IFN-I). Infections and IFN-I treatment were performed in parallel between WT BMDMs and BMDMs lacking genes encoding interferon-responsive factor (IRF) transcription factors, including IRF1, IRF5, and double mutant cells lacking both IRF3 and IRF7. Control cells were uninfected and not treated with IFN-I. The abundance of transcripts between these cells can be compared in order to identify genes specifically regulated by each IRF upon IFN-I treatment.

2019-07-04 | GSE128211 | GEO

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