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ABSTRACT:
PROVIDER: PRJNA542757 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This project focused on the identification of membrane proteins involved in salinity tolerance in the halophyte Salicornia bigelovii. Salicornia bigelovii plants were treated with 0, 50, 200, and 600 mM NaCl for 6 weeks. Membrane preparations for each treatment were obtained in triplicates and were separated into 12 fractions per sample through a continuous sucrose gradient. Based on protein relative abundances per fraction, organellar membrane proteomes were generated. Protein abundances were also compared across treatments.
2024-04-09 | PXD051103 | Pride
Project description:Thellungiella, an Arabidopsis-related halophyte, is an emerging model species for studies designed to elucidate molecular mechanisms of abiotic stress tolerance. Using a cDNA microarray containing 3628 unique sequences derived from previously reported libraries of stress-induced cDNAs of the Yukon ecotype of Thellungiella, we obtained transcript profiles of its response to drought, cold, high salinity and re-watering after drought. A total of 153 transcripts were found to be significantly differentially regulated under the conditions studied. Only six of these genes responded to all three stresses of drought, cold and salinity. Unlike in Arabidopsis, there were relatively few transcript changes in response to high salinity in this halophyte. Furthermore, drought responsive-transcripts in Thellungiella provided a link between the down-regulation of defense-related transcripts and the increase of endogenous abscisic acid during drought. This antagonistic interaction between drought and biotic stress response may potentially be beneficial for survival under drought stress. Intriguingly, changes of transcript abundance in response to cold implicate the involvement of jasmonic acid in the cold acclimation of Thellungiella. Taken together, our results provide useful starting points for more in depth analysis of Thellungiella’s extreme stress tolerance. Keywords: Abiotic stress response
2006-07-22 | GSE2981 | GEO
Project description:This project constructed a multiomics (proteome, metabolome, and transcriptome) database on the response of purslane (Portulaca oleracea L.) plants to salt stress and subsequently started to employ Single-omics (SOA) and Multi-omics Integration (MOI) strategies to characterize the molecular basis of the resistance to salinity stress found in this halophyte species. After evaluating the morpho-physiological responses of purslane plants to salinity stress using a robust salinity stress protocol developed in-house, leaves, and roots were used to generate the database. The proteome data from five plants (control and salt-stressed - leaf and root) was generated and then submitted to the MaxQuant software version 2.1.3.0 for protein identification and abundance, generating a .txt file named “proteinsgroups” that underwent statistical analysis in Perseus software version 2.0.5.0. Here the data and results of the proteome part of the project are presented. For additional information, please read: Silva, V. N. B., da Silva, T. L. C., Ferreira, T. M. M., Neto, J. C. R., Leão, A. P., de Aquino Ribeiro, J. A., Abdelnur, P. V., Valadares, L. F., de Sousa, C. A. F., & Júnior, M. T. S. (2022). Multi-omics Analysis of Young Portulaca oleracea L. Plants' Responses to High NaCl Doses Reveals Insights into Pathways and Genes Responsive to Salinity Stress in this Halophyte Species. Phenomics (Cham, Switzerland), 3(1), 1–21. https://doi.org/10.1007/s43657-022-00061-2
2023-06-01 | PXD041628 | Pride
Project description:This project constructed a multiomics (proteome, metabolome, and transcriptome) database on the response of purslane (Portulaca oleracea L.) plants to salt stress and subsequently started to employ Single-omics (SOA) and Multi-omics Integration (MOI) strategies to characterize the molecular basis of the resistance to salinity stress found in this halophyte species. After evaluating the morpho-physiological responses of purslane plants to salinity stress using a robust salinity stress protocol developed in-house, leaves, and roots were used to generate the database. The proteome data from five plants (control and salt-stressed - leaf and root) was generated and then submitted to the MaxQuant software version 2.1.3.0 for protein identification and abundance, generating a .txt file named “proteinsgroups” that underwent statistical analysis in Perseus software version 2.0.5.0. Here the data and results of the proteome part of the project are presented. For additional information, please read: Silva, V. N. B., da Silva, T. L. C., Ferreira, T. M. M., Neto, J. C. R., Leão, A. P., de Aquino Ribeiro, J. A., Abdelnur, P. V., Valadares, L. F., de Sousa, C. A. F., & Júnior, M. T. S. (2022). Multi-omics Analysis of Young Portulaca oleracea L. Plants' Responses to High NaCl Doses Reveals Insights into Pathways and Genes Responsive to Salinity Stress in this Halophyte Species. Phenomics (Cham, Switzerland), 3(1), 1–21. https://doi.org/10.1007/s43657-022-00061-2
2023-06-01 | PXD041627 | Pride
Project description:Backgroud: microRNA (miRNA) is implicated in plant development processes, playing pivotal roles in plant adaptation to environmental stresses. Salicornia europaea, a salt mash euhalophyte, is a good model plant to study salt adaptation mechanisms. It is also attractive in being vegetables, forage and oilseed that can be used for saline land reclamation and biofuel precursor production on marginal lands. However, none of the miRNAs from S. europaea have been identified so far. Results: Deep sequencing was performed to investigate small RNA transcriptome of S. europaea. Two hundred and twelve conserved miRNAs comprising 51 families and 31 novel miRNAs (including 7 miRNA star sequences) belonging to 30 families were identified. Interestingly, about half (13 out of 31) of the novel miRNAs were only detected in salt-treated samples. The expression of 43 conserved and 13 novel miRNAs changed significantly in response to salinity. In addition, 53 conserved miRNAs and 13 novel miRNAs were differentially expressed between shoots and roots. Furthermore, a total of 306 and 195 S. europaea unigenes were predicted to be targets of 41 conserved and 29 novel miRNA families, respectively. These targets encode a wide range of proteins, and genes involved in transcription regulation constitute the largest category. Four of them, which encode laccase, F-box family protein, SAC3/GANP family protein, and nadph-cytochrome P450 oxydoreductase, were validated using 5'-RACE. Conclusions: Our results indicate specific miRNAs are tightly regulated by salinity in shoots and/or roots of S. europaea, which play important roles in salt adaptation of this euhalophyte. The S. europaea salt-responsive miRNAs and miRNAs that target transcription factors, nucleotide binding site-leucine-rich repeat proteins and enzymes involved in lignin biosynthesis as well as carbon and nitrogen metabolism may be applied in genetic engineering of crops with higher stress tolerance, and genetic modification of biofuel crops with higher biomass and regulatable lignin biosynthesis.
2017-02-09 | GSE62521 | GEO
Project description:We charactarize the regulated miRNAs of Prunus persica to preventive treatment of PpPep2 1 µM endogenous peptide. The peptide treatment was applied to leaves and we sequenced the miRNAs after 1 and 24 h using untreated plants as control. miRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500. Differential expression analysis of annotated pre_miRNA gene biotypes was performed with R package DESeq2 v1.26.0, considering significant epression values below FDR <0.05. We obtained 117,267,647 raw reads and we identified a total of 33 differential expressed miRNAs (DEM) belonging to 18 miRNA families upon PpPep2 application, accounting for 15% of the P. persica annotated miRNAs. miRNA regulation occurs principally one day after peptide treatment. The predicted mRNA targets and function of orthologous miRNAs are compatible with a regulation of PTI-related processes. Together with the transcriptomic response of P. persica to PpPep2 described at our previous RNA-Seq (Foix et al 2021), this suggests that miRNAs would have an active role in PTI regulation at these stages.
2024-12-19 | GSE214135 | GEO
Project description:In order to reveal so far unknown facets of the adaptation of B. subtilis to growth under high-salinity conditions, a whole-transcriptome analysis of B. subtilis BSB (168 Trp+) was performed using strand-specific tiling arrays (tiling step of 22 nucleotides). In addition, the effects of glycine betaine (GB) were analyzed under high salinity and standard growth conditions in a chemically defined medium. Important novel findings were a sustained low-level induction of the SigB-dependent general stress response and strong repression of biofilm matrix genes under high-salinity conditions. GB influences gene expression not only under high-salinity, but also under standard growth conditions without additional salt.
2020-05-18 | GSE145124 | GEO