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RNAseq of Extracellular vesicle RNAs and cellular RNAs from HPASMCs with or without TGF-b1 or BMP4 treatment

ABSTRACT: RNAseq of Extracellular vesicle RNAs and cellular RNAs from HPASMCs with or without TGF-b1 or BMP4 treatment

PROVIDER: PRJNA545533 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9163550_1.fastq.gz	Fastqsanger.gz
	SRR9163550_2.fastq.gz	Fastqsanger.gz
	SRR9163560_1.fastq.gz	Fastqsanger.gz
	SRR9163560_2.fastq.gz	Fastqsanger.gz
	SRR9163551_1.fastq.gz	Fastqsanger.gz

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RNAseq of Extracellular vesicle RNAs and cellular RNAs from HPASMCs with or without TGF-b1 or BMP4 treatment

Project description:Excessive TGF-β signalling has been shown to underlie pulmonary hypertension (PAH). Human pulmonary artery smooth muscle cells (HPASMCs) can release extracellular vesicles (EVs) but their content and significance have not yet been studied. Here, we analysed the content and biological relevance of HPASMC-derived EVs. We used low-input RNA-Seq to analyse the RNAs of EVs released by HPASMC under basal conditions. This data was compared to cellular RNAs. The same experiments (RNA-seq of EV and cellular RNAs) were carried out on HPASMC treated with TGF-b1 and BMP4, recapitulating pathological conditions.

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