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Gene expression changes during EpiLC differentiation in embryonic stem cells that are unable to express Tcf15

ABSTRACT: Gene expression changes during EpiLC differentiation in embryonic stem cells that are unable to express Tcf15

PROVIDER: PRJNA545611 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9164860_1.fastq.gz	Fastqsanger.gz
	SRR9164860_2.fastq.gz	Fastqsanger.gz
	SRR9164861_1.fastq.gz	Fastqsanger.gz
	SRR9164861_2.fastq.gz	Fastqsanger.gz
	SRR9164862.fastq.gz	Fastqsanger.gz

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Gene expression changes during EpiLC differentiation in embryonic stem cells that are unable to express Tcf15

Project description:We analyzed the gene expression changes occuring at the first 24h of EpiLC differentiation in embryonic stem cells in which a regulatory region driving expression of Tcf15 has been deleted. These changes has been compared with wild-type embryonic stem cells.

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WGBS WT J1 ESC at D3 of EpiLC differentiation

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Project description:During in vitro differentiation, pluripotent stem cells undergo extensive remodeling of their gene expression profiles. While studied extensively at the transcriptome level, much less is known about protein dynamics, which might differ significantly from their mRNA counterparts. Here, we present deep proteome-wide measurements of protein levels during the differentiation of embryonic stem cells.

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Project description:DDX6 is an RNA helicase and involved in various post-transcriptional regulatory processes. Although DDX6 is an evolutionarily well conserved central player in post-transcriptional gene regulation, its function in embryonic development remains obscure. In this study, we have identified the crucial functions of DDX6 during early mouse embryogenesis. However, due to its wide range of involvement, the more detailed dissection of DDX6-mediated functions was required to pinpoint which specific DDX6 function is important. For this purpose, we selected a key gene of each well-known DDX6 pathway, generated KO ES line, then made cDNA libraries of ESC and EpiLC Day2 samples to investigate transcriptomic differences during the very early differentiation processes of embryonic pluripotent cells.

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