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The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [RNA-Seq rescue_SS]

ABSTRACT: The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [RNA-Seq rescue_SS]

PROVIDER: PRJNA548149 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9260620_1.fastq.gz	Fastqsanger.gz
	SRR9260620_2.fastq.gz	Fastqsanger.gz
	SRR9260621_1.fastq.gz	Fastqsanger.gz
	SRR9260621_2.fastq.gz	Fastqsanger.gz
	SRR9260622_1.fastq.gz	Fastqsanger.gz

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The RNA exosome nuclease complex regulates human embryonic stem cell differentiation

Project description:The RNA exosome nuclease complex regulates human embryonic stem cell differentiation

| PRJNA548151 | ENA

The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [RNA-Seq SS]

Project description:The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [RNA-Seq SS]

| PRJNA548150 | ENA

The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [PAR-iCLIP]

Project description:The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [PAR-iCLIP]

| PRJNA548148 | ENA

The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [HITS-CLIP]

Project description:The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [HITS-CLIP]

| PRJNA548143 | ENA

The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [PAR-iCLIP]

Project description:This work shows that the exosome modulates the levels of LINE-1 retrotransposons, miRNAs, lncRNAs and specific mRNAs that encode developmental regulators or affect their expression. The exosome restrains differentiation in part by degrading transcripts encoding FOXH1, a transcription factor crucial for mesendoderm formation.

2019-07-11 | GSE132466 | GEO

The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [HITS-CLIP]

2019-07-11 | GSE132360 | GEO

The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [RNA-Seq rescue_SS]

2019-07-11 | GSE132467 | GEO

The RNA exosome nuclease complex regulates human embryonic stem cell differentiation [RNA-Seq SS]

2019-07-11 | GSE132468 | GEO

The RNA exosome nuclease complex regulates human embryonic stem cell differentiation.

Project description:A defining feature of embryonic stem cells (ESCs) is the ability to differentiate into all three germ layers. Pluripotency is maintained in part by a unique transcription network that maintains expression of pluripotency-specific transcription factors and represses developmental genes. While the mechanisms that establish this transcription network are well studied, little is known of the posttranscriptional surveillance pathways that degrade differentiation-related RNAs. We report that the surveillance pathway mediated by the RNA exosome nuclease complex represses ESC differentiation. Depletion of the exosome expedites differentiation of human ESCs into all three germ layers. LINE-1 retrotransposons and specific miRNAs, lncRNAs, and mRNAs that encode developmental regulators or affect their expression are all bound by the exosome and increase in level upon exosome depletion. The exosome restrains differentiation in part by degrading transcripts encoding FOXH1, a transcription factor crucial for mesendoderm formation. Our studies establish the exosome as a regulator of human ESC differentiation and reveal the importance of RNA decay in maintaining pluripotency.

| S-EPMC6683745 | biostudies-literature

The fission yeast MTREC complex targets CUTs and unspliced mRNAs to the nuclear exosome [RNA-Seq]

Project description:Cryptic unstable transcripts (CUTs) are rapidly degraded by the nuclear exosome, however, the way they are recognized and targeted to the exosome is not fully understood. The recently identified Schizosaccharomyces pombe MTREC complex has been shown to promote degradation of meiotic mRNAs and regulatory ncRNAs. Here, we report that the MTREC complex is also the major nuclear exosome targeting complex for CUTs and unspliced mRNAs. MTREC complex specifically binds to CUTs, meiotic mRNAs and unspliced mRNA transcripts and targets these RNAs for degradation by the nuclear exosome, while the TRAMP complex has only a minor role in this process. The MTREC complex physically interacts with the nuclear exosome and with various RNA-binding and -processing complexes, coupling RNA processing to the RNA degradation machinery. Our study reveals the central role of the evolutionarily conserved MTREC complex in RNA quality control, and in the recognition and elimination of aberrant, cryptic transcripts. RNA sequencing of WT and mutant S.pombe strains, processed data is normalized to median non-intron containing gene-expression, no replicates

2015-05-22 | E-GEOD-64991 | biostudies-arrayexpress

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