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Chlorella variabilis NC64A and Chlorovirus PBCV-1

ABSTRACT: The feedback between selection and demography shapes genomic diversity during coevolution

PROVIDER: PRJNA548271 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9640550_1.fastq.gz	Fastqsanger.gz
	SRR9640550_2.fastq.gz	Fastqsanger.gz
	SRR9640560_1.fastq.gz	Fastqsanger.gz
	SRR9640560_2.fastq.gz	Fastqsanger.gz
	SRR9640570_1.fastq.gz	Fastqsanger.gz

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Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis.

Project description:Chloroviruses infect their hosts by specifically binding to and degrading the cell wall of their algal hosts at the site of attachment, using an intrinsic digesting enzyme(s). Chlorovirus PBCV-1 stored as a lysate survived longer than virus alone, suggesting virus attachment to cellular debris may be reversible. Ghost cells (algal cells extracted with methanol) were used as a model to study reversibility of PBCV-1 attachment because ghost cells are as susceptible to attachment and wall digestion as are live cells. Reversibility of attachment to ghost cells was examined by releasing attached virions with a cell wall degrading enzyme extract. The majority of the released virions retained infectivity even after re-incubating the released virions with ghost cells two times. Thus the chloroviruses appear to have a dynamic attachment strategy that may be beneficial in indigenous environments where cell wall debris can act as a refuge until appropriate host cells are available.

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Global analysis of Chlorella variabilis NC64A mRNA profiles during the early phase of Paramecium bursaria chlorella virus-1 infection.

Project description:The PBCV-1/Chlorella variabilis NC64A system is a model for studies on interactions between viruses and algae. Here we present the first global analyses of algal host transcripts during the early stages of infection, prior to virus replication. During the course of the experiment stretching over 1 hour, about a third of the host genes displayed significant changes in normalized mRNA abundance that either increased or decreased compared to uninfected levels. The population of genes with significant transcriptional changes gradually increased until stabilizing at 40 minutes post infection. Functional categories including cytoplasmic ribosomal proteins, jasmonic acid biosynthesis and anaphase promoting complex/cyclosomes had a significant excess in upregulated genes, whereas spliceosomal snRNP complexes and the shikimate pathway had significantly more down-regulated genes, suggesting that these pathways were activated or shut-down in response to the virus infection. Lastly, we examined the expression of C. varibilis RNA polymerase subunits, as PBCV-1 transcription depends on host RNA polymerases. Two subunits were up-regulated, RPB10 and RPC34, suggesting that they may function to support virus transcription. These results highlight genes and pathways, as well as overall trends, for further refinement of our understanding of the changes that take place during the early stages of viral infection.

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Chlorella variabilis strain:NC64A

Project description:Chlorella variabilis

| PRJNA45853 | ENA

Chlorella variabilis strain:NC64A

Project description:Chlorella variabilis Transcriptome during infection by PBCV-1

| PRJNA210187 | ENA

Evaluation of higher plant virus resistance genes in the green alga, Chlorella variabilis NC64A, during the early phase of infection with Paramecium bursaria chlorella virus-1.

Project description:With growing industrial interest in algae plus their critical roles in aquatic systems, the need to understand the effects of algal pathogens is increasing. We examined a model algal host-virus system, Chlorella variabilis NC64A and virus, PBCV-1. C. variabilis encodes 375 homologs to genes involved in RNA silencing and in response to virus infection in higher plants. Illumina RNA-Seq data showed that 325 of these homologs were expressed in healthy and early PBCV-1 infected (?60min) cells. For each of the RNA silencing genes to which homologs were found, mRNA transcripts were detected in healthy and infected cells. C. variabilis, like higher plants, may employ certain RNA silencing pathways to defend itself against virus infection. To our knowledge this is the first examination of RNA silencing genes in algae beyond core proteins, and the first analysis of their transcription during virus infection.

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Chlorella variabilis strain:NC64A (ATCC 50258)

Project description:De novo assembly of middle-sized genome of symbiotc algae Chlorella variabilis using MinION and Illumina sequencers

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Chlorella variabilis strain:NC64A (ATCC 50528)

Project description:Multi-omics analysis of the symbiotic green algae, Chlorella variabilis, revealing the genetic basis of the obligate endosymbiotic lifestyle

| PRJDB12190 | ENA

Chlorella variabilis

Project description:freshwater, unicellular green alga

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Chlorella variabilis

Project description:Chlorella variabilis Raw sequence reads

| PRJNA550224 | ENA

Genome wide RNA-Seq of LOG OX1 and wildtype Chlorella variabilis cultures

Project description:To assess the significant downstream pathways affected by the overexpression of LONELY GUY in Chlorella, transcriptome analysis was carried out using RNA sequencing of three biological replicates each from WT and LOG OX1 at 14 dpi (1h before dark phase). The 14 dpi was chosen for the analysis because it was in the middle of the growth phase, where the slower growth of LOG OX1 was evident.

2021-02-24 | GSE162985 | GEO

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