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Transcriptome data after TRIM28 knockdown in A375 melanoma cells

ABSTRACT: Transcriptome data after TRIM28 knockdown in A375 melanoma cells

PROVIDER: PRJNA549885 | ENA |

REPOSITORIES: ENA

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Transcriptome data after TRIM28 knockdown in A375 melanoma cells

Project description:TRIM28 is a bromodomain protein implicated in the regulation of tumor growth. TRIM28 regulates gene expression by recruiting epigenetic writers including HDACs, SETDB1 and HP1. To determine the role of TRIM28 in melanoma cells we knocked down TRIM28 using lentiviral shRNA constructs followed by gene expression analysis using HTA 2.0 GeneChip.

2019-06-21 | GSE133073 | GEO

Expression data from A375 melanoma parental and persister cells

Project description:Identify transcriptionnally and translationally regulated mRNA in melanoma parental and persister cells In this dataset, we include expression data of A375 melanoma drug-naïve parental cells and A375 melanoma persister cells that survived from BRAF and MEK inhibition. The expression data are studied in both total RNA and polysome-bounded RNA.

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A375 melanoma cells treated with leflunomide 25uM

Project description:Human A375 melanoma cells were treated with leflunomide 25uM for 3 days, then RNA harvested 6 arrays

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Expression data from BRAFV600E A375 melanoma cells treated with vehicle or vemurafenib

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2014-05-20 | E-GEOD-42872 | biostudies-arrayexpress

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Project description:Human A375 melanoma cells were treated with leflunomide 25uM for 3 days, then RNA harvested

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NFATC2 knock-down on melanoma cell line A375

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The bromodomain protein TRIM28 controls the balance between growth and invasiveness in melanoma

Project description:Melanoma tumors are highly metastatic partly due to the ability of melanoma cells to transition between invasive and proliferative states, however, the mechanisms underlying this plasticity are still not fully understood. To identify new epigenetic regulators of melanoma plasticity, we combined data mining, tumor models, proximity proteomics, and CUT&RUN-sequencing. We focused on the druggable family of bromodomain epigenetic readers, and identified TRIM28 as a new regulator of melanoma plasticity. We found that TRIM28 promotes the expression of pro-invasive genes, and that TRIM28 controls the balance between invasiveness and growth of melanoma cells. We demonstrate that TRIM28 acts via the transcription factor JUNB, that directly regulates the expression of pro-invasive and pro-growth genes. Mechanistically, TRIM28 controls the expression of JUNB by negatively regulating its transcriptional elongation by RNA polymerase II. In conclusion, our results demonstrate that a TRIM28-JUNB axis controls the balance between invasiveness and growth in melanoma tumors and suggests that the bromodomain protein TRIM28 could be targeted to reduce tumor spread.

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Gene expression changes in A375 melanoma cells in response to ERK3 knockdown.

Project description:Gene expression changes in A375 melanoma cells in response to ERK3 knockdown.

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Transcriptome-wide map of m6A RNAs in A375 NC cells and shMETTL3 cells

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