Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Neocortical layer 4 in adult mouse differs in major cell types and circuit organization between primary sensory areas

ABSTRACT: Neocortical layer 4 in adult mouse differs in major cell types and circuit organization between primary sensory areas

PROVIDER: PRJNA554897 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9694280.fastq.gz	Fastqsanger.gz
	SRR9694290.fastq.gz	Fastqsanger.gz
	SRR9694300.fastq.gz	Fastqsanger.gz
	SRR9694310.fastq.gz	Fastqsanger.gz
	SRR9694320.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 118

Similar Datasets

Neocortical layer 4 in adult mouse differs in major cell types and circuit organization between primary sensory areas

Project description:Layer 4 (L4) of mammalian neocortex plays a crucial role in cortical information processing, yet a complete census of its cell types and connectivity remains elusive. Using whole-cell recordings with morphological recovery, we identified one major excitatory and seven inhibitory types of neurons in L4 of adult mouse visual cortex (V1). Nearly all excitatory neurons were pyramidal and all somatostatin-positive (SOM+) non-fast-spiking interneurons were Martinotti cells. In contrast, in somatosensory cortex (S1), excitatory neurons were mostly stellate and SOM+ interneurons were non-Martinotti. These morphologically distinct SOM+ interneurons corresponded to different transcriptomic cell types and were differentially integrated into the local circuit with only S1 neurons receiving local excitatory input. We propose that cell type specific circuit motifs, such as the Martinotti/pyramidal and non-Martinotti/stellate pairs, are used across the cortex as building blocks to assemble cortical circuits.

2019-08-06 | GSE134378 | GEO

Layer 4 of mouse neocortex differs in cell types and circuit organization between sensory areas.

| S-EPMC6744474 | biostudies-literature

Shared and distinct transcriptomic cell types across neocortical areas

Project description:Shared and distinct transcriptomic cell types across neocortical areas

| PRJNA476008 | ENA

Cell type composition and circuit organization of neocortical radial clones

Project description:Cell type composition and circuit organization of neocortical radial clones

| PRJNA591575 | ENA

Cell type composition and circuit organization of neocortical radial clones

Project description:Excitatory neurons arising from a common progenitor establish radially-oriented clonal units in the neocortex which have been proposed to serve as elementary information processing modules. To characterize the cell types and circuit diagram within these clonal units, we performed single-cell RNA-sequencing and multi-cell patch clamp recordings of neurons derived from Nestin-positive progenitors. We found that radial clones do not appear to be fate-restricted, but instead individual clones are composed of a random sampling of the transcriptomic cell types present in a particular cortical area. The effect of lineage on synaptic connectivity depends on the type of connection tested: pairs of clonally related neurons were more likely to be connected vertically, across cortical layers, but not laterally within the same layer, compared to unrelated pairs. We propose that integration of vertical input from related neurons with lateral input from unrelated neurons may represent a developmentally programmed motif for assembling neocortical circuits.

2020-03-05 | GSE140946 | GEO

Neocortical projection neurons instruct inhibitory interneuron circuit development in a lineage dependent manner

Project description:Neocortical projection neurons instruct inhibitory interneuron circuit development in a lineage dependent manner

| PRJNA525662 | ENA

Layer-specific molecular expression in neocortical astrocytes

Project description:Layer-specific molecular expression in neocortical astrocytes

| PRJNA438534 | ENA

Neocortical projection neurons instruct inhibitory interneuron circuit development in a lineage dependent manner

Project description:Neocortical circuits consist of stereotypical motifs that must self-assemble during development. Recent evidence suggests the subtype identity of both excitatory projection neurons (PNs) and inhibitory interneurons (INs) is important for this process. We knocked out the transcription factor Satb2 in PNs to induce those of the intratelencephalic (IT)-type to adopt a pyramidal tract (PT)-type identity. Loss of IT-type PNs selectively disrupted the lamination and circuit integration of INs derived from the caudal ganglionic eminence (CGE). Strikingly, reprogrammed PNs demonstrated reduced synaptic targeting of CGE-derived INs relative to controls. In control mice, IT-type PNs targeted neighboring CGE INs while PT-type PNs did not in deep layers, confirming this lineage-dependent motif. Finally, single cell RNA-sequencing revealed that major CGE IN subtypes were conserved after loss of IT PNs, but with differential transcription of synaptic proteins and signaling molecules. Thus, IT-type PNs influence CGE-derived INs in a non-cell autonomous manner during cortical development.

2019-03-06 | GSE127724 | GEO

Transcription profiling of mouse neocortical and cerebellar astrocytes

Project description:Affymetrix Mouse Genome 430 2.0 GeneChip microarrays were used to analyze murine neocortical and cerbellar astrocytes generated from postnatal (PN) day 1 wild-type (ICR) pups. Experiment Overall Design: Three samples each of murine neocortical and cerebellar astrocytes were analyzed.

2008-06-13 | E-GEOD-5582 | biostudies-arrayexpress

Neocortical and cerebellar astrocytes

Project description:Affymetrix Mouse Genome 430 2.0 GeneChip microarrays were used to analyze murine neocortical and cerbellar astrocytes generated from postnatal (PN) day 1 wild-type (ICR) pups. Keywords: neocortical astrocyte, cerebellar astrocyte, murine, postnatal day 1

2007-03-14 | GSE5582 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data