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Expression profiles of satellite cells and alpha7Sca1 cells in mdxITGAM-DTR mice

ABSTRACT: Expression profiles of satellite cells and alpha7Sca1 cells in mdxITGAM-DTR mice

PROVIDER: PRJNA556367 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9822580.fastq.gz	Fastqsanger.gz
	SRR9822581.fastq.gz	Fastqsanger.gz
	SRR9822576.fastq.gz	Fastqsanger.gz
	SRR9822577.fastq.gz	Fastqsanger.gz
	SRR9822578.fastq.gz	Fastqsanger.gz

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Expression profiles of satellite cells and alpha7Sca1 cells in mdxITGAM-DTR mice

Project description:We took advantage of a dystrophic mouse model of transient macrophage-depletion, mdxITGAM-DTR mice, in order to analyze the role of macrophage in skeletal muscle regeneration. We generated the transcriptome of satellite cells (SCs) and alpha7Sca1 cells purified by cell sorting from mdxITGAM-DTR mice. The mice were treated, by intramuscular injection, with PBS, as vehicle, or with Diphtheria toxin (DT) in order to achieve the macrophage depletion form hind-limb muscle We described a shift in identity of muscle stem cells dependent on the crosstalk between macrophages and satellite cells. Indeed macrophage depletion determines an exacerbated dystrophic phenotype associated with adipogenic conversion of SCs and reduction of the SC pool.

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Gene expression profiles in skeletal muscle satellite cells lacking Dnmt1

Project description:To identify the target genes of Dnmt1 in skeletal muscle satellite cells, we generated satellite cell specific Dnmt1 KO using Pax7-CreERT2 and Dnmt1 flox mice. After Tamoxifen (cKO) or vehicle (Control) treatment for 5 consecutive days, mice were euthanized and muscle satellite cells were collected by FACS sorting and total RNA were extracted.

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