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Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

ABSTRACT: Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

PROVIDER: PRJNA556421 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9824440.fastq.gz	Fastqsanger.gz
	SRR9824441.fastq.gz	Fastqsanger.gz
	SRR9824442.fastq.gz	Fastqsanger.gz
	SRR9824433.fastq.gz	Fastqsanger.gz
	SRR9824443.fastq.gz	Fastqsanger.gz

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Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

Project description:Overexpression of activin A by keratinocytes accelerates excisional wound healing in mice. Activin-promoted wound healing is mediated via the stroma, specifically by the wound immune cells and fibroblasts. To determine if activin A alters the gene expression profile of wound fibroblasts, we performed RNA-sequencing of fibroblasts FACS-sorted from excisional skin wounds of activin overexpressing mice. We found that activin induces a pro-fibrotic gene expression profile of these fibroblasts, leading to the upregulation of genes involved in collagen biosynthesis and remodeling.

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Analysis of the expression profile of skin fibroblasts FACS-sorted from mice overexpressing activin and/or oncogenes of human papilloma virus 8 in keratinocytes.

Project description:Overexpression of activin by keratinocytes promotes HPV8-induced skin tumorigenesis in mice. Activin-promoted tumorigenesis is mediated via the stroma, specifically by cancer-associated fibroblasts (CAFs). To determine if activin alters the gene expression profile of skin fibroblasts, we performed RNA-sequencing of fibroblasts FACS-sorted from pre-cancerous ear skin of activin overexpressing mice. We found that activin induces a CAF-like gene expression profile of fibroblasts, leading to the upregulation of genes involved in proliferation, migration and cytoskeletal remodeling.

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Analysis of the expression profile of skin macrophages FACS-sorted from mice overexpressing activin and/or oncogenes of human papilloma virus 8 in keratinocytes.

Project description:We have shown that activin promoted skin tumorigenesis in mice induced by the human papilloma virus 8 oncogenes. Activin attracted blood monocytes to the skin as revealed by depletion of CCR2-positive monocytes. To determine if activin also altered the gene expression profile of these cells, we performed RNA-Sequencing of macrophages FACS-sorted from the pre-cancerous ear skin. We have found that activin induces a pro-migratiory, pro-angiogenic and pro-tumorigenic genes in skin macrophages in vivo. This largely contributes to the pro-tumorigenic function of activin, since macrophage depletion delayed spontaneous tumorigenesis in HPV8-transgenic mice by reducing keratinocyte proliferation and angiogenesis.

2017-02-08 | GSE79086 | GEO

Analysis of the expression profiles of back skin and spontaneous back skin tumor-derived fibroblasts FACS-sorted from mice overexpressing oncogenes of human papilloma virus 8 in keratinocytes.

Project description:Overexpression of the oncogene HPV8 in keratinocytes of mice causes spontaneous papillomas to form in the skin. To study the expression profiles of skin cancer-associated fibroblasts, we performed RNA-sequencing of fibroblasts FACS-sorted from back skin tumors of HPV8 overexpressing mice.

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Project description:FACS sorted glomerular kidney cells from a single mouse were subjected to proteomics.

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Analysis of the expression profiles of back skin and spontaneous back skin tumor-derived fibroblasts FACS-sorted from mice overexpressing oncogenes of human papilloma virus 8 in keratinocytes.

Project description:Analysis of the expression profiles of back skin and spontaneous back skin tumor-derived fibroblasts FACS-sorted from mice overexpressing oncogenes of human papilloma virus 8 in keratinocytes.

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Project description:The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with proteomics can be used to sort and analyze cell populations based on their metabolic state. A previously established GFP reporter system was used to detect and sort single Corynebacterium glutamicum cells based on the concentration of branched chain amino acids (BCAA) using FACS. A proteomics workflow optimized for small cell numbers was used to quantitatively compare proteomes of a ?aceE mutant, lacking functional pyruvate dehydrogenase (PD), and the wild type. About 800 proteins could be quantified from 1,000,000 cells. In the ?aceE mutant BCAA production was coordinated with upregulation of the glyoxylate cycle and TCA cycle to counter the lack of acetyl CoA resulting from the deletion of aceE.

2017-11-02 | PXD005812 | Pride

Comparing the transcriptome of early and late phase wound macrophages in skin

Project description:We inflicted full thickness excisional wounds in the back skin of C57BL/6 wild-type mice. Injury-associated macrophages were isolated from wound tissue during the early inflammatory phase (4 days post injury) and in the late, scar forming phase (14 days post injury), sorted by flow cytometry (CD45+CD11b+F4/80+ wound cells), and subjected to RNAsequencing.

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Project description:To determine the role of the cytokine activin A in the regulation of human T follicular helper (Tfh) cell gene program, we performed a transcriptomic analysis (RNA-seq) of human naÃ¯ve CD4 T cells differentiated in vitro with activin A. The analysis of the gene expression profile driven by activin A, alone or in combination with IL-12 (a know regulator of human Tfh differentiation/function), revealed that activin A can regulate the expression of multiple molecules involved in the differentiation and/or function of human Tfh cells. Human naÃ¯ve CD4 T cells were isolated from fresh PBMCs of healthy control subjects by magnetic bead isolation. Purity was measured by FACS as percentage of CD4+CD45RA+ cells and was 95% or higher. Upon isolation, naÃ¯ve CD4 T cells were stimulated with anti-CD3/CD28 coated beads in the presence of the following cytokine combinations: no exogenous cytokines (beads only), activin A, IL-12, activin A+IL-12, TGFb, TGFb +IL12. Following 5 days of in vitro culture, live CD4 T cells were FACS sorted and gene expression was analyzed by RNA-seq. Data are from independent donors.

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