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Expression profiles of long noncoding RNAs and mRNAs in A549 cells infected by Influenza virus

ABSTRACT: Expression profiles of long noncoding RNAs and mRNAs in A549 cells infected by Influenza virus

PROVIDER: PRJNA559077 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9915563_1.fastq.gz	Fastqsanger.gz
	SRR9915563_2.fastq.gz	Fastqsanger.gz
	SRR9915564_1.fastq.gz	Fastqsanger.gz
	SRR9915564_2.fastq.gz	Fastqsanger.gz
	SRR9915565_1.fastq.gz	Fastqsanger.gz

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Expression profiles of long noncoding RNAs and mRNAs in A549 cells infected by Influenza virus

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Analysis of Expression Profiles of long noncoding RNAs in A549 cells infected by H1N1 [lncRNA-seq]

Project description:Although accumulating evidence has shown that long non-coding RNAs (lncRNAs) are involved in multiple biological processes, considerably less is known regarding their functions in influenza A virus (IAV) replication. Here, lncRNA expression profiles were determined by RNA sequencing in three pairs of influenza virus A/Puerto Rico/8/34 (H1N1)-infected or uninfected A549 cells.

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Analysis of Expression Profiles of Long Noncoding RNAs and mRNAs in A549 Cells Infected with H3N2 Swine Influenza Virus by RNA Sequencing.

Project description:Long noncoding RNAs (lncRNAs) participate in regulating many biological processes. However, their roles in influenza A virus (IAV) pathogenicity are largely unknown. Here, we analyzed the expression profiles of lncRNAs and mRNAs in H3N2-infected cells and mock-infected cells by high-throughput sequencing. The results showed that 6129 lncRNAs and 50,031 mRNA transcripts in A549 cells displayed differential expression after H3N2 infection compared with mock infection. Among the differentially expressed lncRNAs, 4963 were upregulated, and 1166 were downregulated. Functional annotation and enrichment analysis using gene ontology and Kyoto Encyclopedia of Genes and Genomes databases (KEGG) suggested that target genes of the differentially expressed lncRNAs were enriched in some biological processes, such as cellular metabolism and autophagy. The up- or downregulated lncRNAs were selected and further verified by quantitative real-time polymerase chain reaction (RT-qPCR) and reverse transcription PCR (RT-PCR). To the best of our knowledge, this is the first report of a comparative expression analysis of lncRNAs in A549 cells infected with H3N2. Our results support the need for further analyses of the functions of differentially expressed lncRNAs during H3N2 infection.

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Differential miRNA expression profiling of A549 cells infected with influenza A virus

Project description:To further understand the roles of miRNA during influenza A virus infection, we performed miRNA profiling in human alveolar adenocarcinoma cell lines, A549 cells, infected with influenza A virus A/Beijing/501/2009(H1N1) and A/goose/Jilin/hb/2003(H5N1).

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Expression profiles of long noncoding RNAs and mRNAs in ST cells infected by PDCoV

Project description:Long noncoding RNAs (lncRNAs) participate in regulating many biological processes. However, their roles in PDCoV pathogenicity are largely unknown. Here, we analyzed the expression profile of lncRNAs and mRNAs in the PDCoV-infected cells by high-throughput sequencing

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Project description:Analysis of gene expression in A549 cells infected with influenza A virus or Mock and treated with Itaconic acid (IA), or Dimethyl itaconate (DI) to investigate the effects itaconate have on transcriptional response in A549 cells. We particularly looked at anti-inflammatory effects of itaconate in inflammation followed by Influenza A virus infection.

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Project description:The temporal response of primary human alveolar adenocarcinoma epithelial cells (A549) infected with H5N1 influenza virus and H9N2 influenza A viruswere evaluated using the proteomics approaches (2D-DIGE combined with MALDI-TOF-MS/MS) at 24 hours post of the infection .

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Gene Expression Data from Influenza A Virus-Infected and Mock-infected A549 Human Aveolar Epithelial Carcinoma Cells with Exposure to Mouse Eosinophils.

Project description:Eosinophils are terminally differentiated granulocytes that participate in innate immunity against Influenza A Virus (IAV). Here, we investigate transcriptional changes that occur in IAV-infected human A549 aveolar epithelial carcinoma cells with and without direct or indirect exposure to mouse bone marrow-derived eosinophils (BMdEos). Global changes in the transcriptome of A549 cells due to Influenza A virus-infection and/or direct and indirect eosinophil exposure were assessed using ThermoFisher/Affymetrix Clariom™ S Human microarrays.

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