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Effect of ILCs depletion on Macrophages in a mouse model of colitis

ABSTRACT: Effect of ILCs depletion on Macrophages in a mouse model of colitis

PROVIDER: PRJNA559088 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9916590_1.fastq.gz	Fastqsanger.gz
	SRR9916590_2.fastq.gz	Fastqsanger.gz
	SRR9916591_1.fastq.gz	Fastqsanger.gz
	SRR9916591_2.fastq.gz	Fastqsanger.gz
	SRR9916592_1.fastq.gz	Fastqsanger.gz

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Effect of ILCs depletion on Macrophages in a mouse model of colitis

Project description:Anti CD90 was used to deplete ILCs in RAG mice at point of iniation of DSS colitis in Rag mice. After 6 days the Macrophages were sorted from the colon and compared by using RNASeq to mice in which ILCs had not been repleted but still had colitus induced.

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Expression of the transcription factor HIF-1α in NKp46+ innate lymphoid cells limits inflammation and fibrosis upon chronic colitis

Project description:Innate lymphoid cells (ILCs) are critically involved in intestinal adaptation to microenvironmental challenges (Colonna, 2018; Guendel et al., 2020) and the gut mucosa is characterized by low oxygen. Adaptation to low oxygen is mediated by Hypoxia-inducible transcription factors (HIFs) the HIF-1α subunit shapes the ILC phenotype upon acute experimental colitis and contributes to intestinal damage. However, the impact of HIF signaling in NKp46+ ILC in the context of repetitive mucosal damage and chronic inflammation, as it typically occurs during inflammatory bowel disease, is unknown. In a model of chronic colitis, mice lacking the HIF-1α isoform in NKp46+ ILCs show a decrease in NKp46+ ILC1s but a concomitant rise in neutrophils as well as Ly6C high macrophages. Single-nuclei RNA sequencing suggests enhanced interaction of mesenchymal cells with other cell compartments in the colon of HIF-1α KO mice and a loss of mucus-producing enterocytes as well as intestinal stem cells. This was, furthermore, associated with increased BMP-integrin signaling, expansion of profibrotic fibroblasts subsets and intestinal fibrosis. In summary this suggests that HIF-1 α mediated ILC1 activation, although detrimental upon acute colitis, protects against excessive inflammation and fibrosis during chronic intestinal damage.

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Single cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs)

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