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Responses to DNA vaccine against H5 virus in White Leghorn SPF and Rosa 1 chickens

ABSTRACT: Responses to DNA vaccine against H5 virus in White Leghorn SPF and Rosa 1 chickens

PROVIDER: PRJNA559613 | ENA |

REPOSITORIES: ENA

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Responses to DNA vaccine against H5 virus in White Leghorn SPF chickens.

Project description:White Leghorn SPF chickens were immunized with two doses of DNA vaccine, based on the hemagglutinin (HA) DNA from H5N1 virus, in comparison to the control group, which was administered an empty vector (pCI). Gene expression changes in the spleens of chickens were investigated at 7 day post booster dose.

2020-02-29 | GSE135669 | GEO

Responses to DNA vaccine against H5 virus in White Leghorn SPF chickens.

Project description:Responses to DNA vaccine against H5 virus in White Leghorn SPF chickens.

| PRJNA559615 | ENA

Responses to DNA vaccine against H5 virus in White Leghorn SPF and Rosa 1 chickens

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-01 | GSE135671 | GEO

Responses to DNA vaccine against H5 virus in spleens of Rosa 1 chickens

Project description:Laying hens Rosa 1 were immunized with two doses of DNA vaccine, based on the hemagglutinin (HA) DNA from H5N1 virus, in comparison to the control group, which was administered an empty vector (pCI). Additional groups of Rosa 1 hens were treated with one dose of above described vaccine or empty vector. Gene expression changes in the spleens of chickens were investigated at 7 day post last vaccination dose.

2020-02-29 | GSE135670 | GEO

Responses to DNA vaccine against H5 virus in spleens of Rosa 1 chickens

Project description:Responses to DNA vaccine against H5 virus in spleens of Rosa 1 chickens

| PRJNA559614 | ENA

Gallus gallus strain:Specified pathogen free (SPF) - white leghorn

Project description:Gallus gallus strain:Specified pathogen free (SPF) - white leghorn Transcriptome or Gene expression

| PRJNA171809 | ENA

Gene expression profile at liver tissue of White Leghorn and four Korean native chickens

Project description:To characterize breed-specific difference among four Korean native chicken breeds and White Leghorn, we measured their transcriptomes at liver tissue using Affymetrix Chicken gene 1.0 ST array platform.

2015-12-31 | GSE63389 | GEO

Evaluation of dendritic cell-targeting T7 phages as a vehicle to deliver avian influenza virus H5 DNA vaccine in SPF chickens.

Project description:IntroductionThere is a growing demand for effective technologies for the delivery of antigen to antigen-presenting cells (APCs) and their immune-activation for the success of DNA vaccines. Therefore, dendritic cell (DC)-targeting T7 phages were used as a vehicle to deliver DNA vaccine.MethodsIn this study, a eukaryotic expression plasmid pEGFP-C1-HA2-AS containing the HA2 gene derived from the avian H5N1 virus and an anchor sequence (AS) gene required for the T7 phage packaging process was developed. To verify the feasibility of phage delivery, the plasmid encapsulated in DC-targeting phage capsid through the recognition of AS was evaluated both in vitro and in vivo. The pEGFP-C1-HA2-AS plasmid could evade digestion by DNase I by becoming encapsulated into the phage particles and efficiently expressed the HA2 antigen in DCs with the benefit of DC-targeting phages.ResultsFor chickens immunized with the DC-targeting phage 74 delivered DNA vaccine, the levels of IgY and IgA antibodies, the concentration of IFN-γ and IL-12 cytokines in serum, the proliferation of lymphocytes, and the percentage of CD4+/CD8+ T lymphocytes isolated from peripheral blood were significantly higher than chickens which were immunized with DNA vaccine that was delivered by non-DC-targeting phage or placebo (p<0.05). Phage 74 delivered one-fiftieth the amount of pEGFP-C1-HA2-AS plasmid compared to Lipofectin, however, a comparable humoral and cellular immune response was achieved. Although, the HA2 DNA vaccine delivered by the DC-targeting phage induced enhanced immune responses, the protection rate of virus challenge was not evaluated.ConclusionThis study provides a strategy for development of a novel avian influenza DNA vaccine and demonstrates the potential of DC-targeting phage as a DNA vaccine delivery vehicle.

| S-EPMC9799975 | biostudies-literature

Succinivibrio dextrinosolvens H5 strain:H5

Project description:Succinivibrio dextrinosolvens H5 strain:H5 Genome sequencing and assembly

| PRJNA223462 | ENA

Enhydrobacter sp. H5 strain:H5

Project description:Enhydrobacter sp. H5 strain:H5 Genome sequencing and assembly

| PRJNA347875 | ENA