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Responses to DNA vaccine against H5 virus in White Leghorn SPF chickens.

ABSTRACT: Responses to DNA vaccine against H5 virus in White Leghorn SPF chickens.

PROVIDER: PRJNA559615 | ENA |

REPOSITORIES: ENA

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Responses to DNA vaccine against H5 virus in White Leghorn SPF chickens.

Project description:White Leghorn SPF chickens were immunized with two doses of DNA vaccine, based on the hemagglutinin (HA) DNA from H5N1 virus, in comparison to the control group, which was administered an empty vector (pCI). Gene expression changes in the spleens of chickens were investigated at 7 day post booster dose.

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Responses to DNA vaccine against H5 virus in White Leghorn SPF and Rosa 1 chickens

Project description:Responses to DNA vaccine against H5 virus in White Leghorn SPF and Rosa 1 chickens

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Next Generation Sequencing Facilitates Quantitative Analysis of H5N1 Wild Type and NS1-truncated Lung Transcriptomes in SPF chickens

Project description:H5N1 highly pathogenic avian influenza (HPAI) has raised global concern for causing huge economic losses in poultry industry. By using reversal genetic technical, we have rescued a H5 wild-type virus rS and its NS1-truncated virus S-NS99, harboring only 99 amino acids in the amino terminus of NS1. We then used a high-throughput RNA-seq method to analyze host and pathogen transcriptomes in the lungs of chickens infected with rS and S-NS99. Sequenced numbers of viral transcripts and expression levels of host immune-related genes at 1 day post infection (dpi) were higher in rS-infected than S-NS99-infected chickens. The numbers of immune response of rS-infected chickens by Gene Ontology (GO) enrichment were higher than S-NS99-infected chickens. KEGG pathway analysis revealed rS infection could provoke significantly stronger immune response than S-NS99. Collectively, our data provide new insight into the underlying mechanisms of the diﬀerential length NS1 of avian influenza viruses.

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Responses to DNA vaccine against H5 virus in spleens of Rosa 1 chickens

Project description:Laying hens Rosa 1 were immunized with two doses of DNA vaccine, based on the hemagglutinin (HA) DNA from H5N1 virus, in comparison to the control group, which was administered an empty vector (pCI). Additional groups of Rosa 1 hens were treated with one dose of above described vaccine or empty vector. Gene expression changes in the spleens of chickens were investigated at 7 day post last vaccination dose.

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