Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next genereration sequencing reveals that CNAs associated with cyclin E overexpression tranlate into changes in transcriptional output.

ABSTRACT: Next genereration sequencing reveals that CNAs associated with cyclin E overexpression tranlate into changes in transcriptional output.

PROVIDER: PRJNA561187 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10007835_1.fastq.gz	Fastqsanger.gz
	SRR10007835_2.fastq.gz	Fastqsanger.gz
	SRR10007836_1.fastq.gz	Fastqsanger.gz
	SRR10007836_2.fastq.gz	Fastqsanger.gz
	SRR10007837_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 26

Similar Datasets

Next genereration sequencing reveals that CNAs associated with cyclin E overexpression tranlate into changes in transcriptional output.

Project description:Transient cyclin E overexpression in human mammary epithelial cells leads to a distinct CNA signature. We carried out high throughput sequencing to determine if these CNAs translated into changes in transcription.

2020-08-30 | GSE136078 | GEO

Cyclin overexpression

Project description:Yeast cells were arrested either in G1 (for CLN3 overexpression) or in G2/M (for CLB2 overexpression). The cyclin was then induced, and samples were taken. This study is described in more detail in Spellman PT et al.(1998) Mol Biol Cell 9:3273-97 Keywords: other

2002-02-12 | GSE25 | GEO

Expression data from JVM13 cells with cyclin D1 exogenous overexpression

Project description:Mantle cell lymphoma (MCL) is an aggressive B-cell neoplasm characterized by the t(11;14)(q13;q32) translocation leading to cyclin D1 overexpression. Cyclin D1 is a major cell cycle regulator and also has a role in transcription, but the effect of the latter in tumorigenesis remains largely unknown. Here, we investigated the transcriptional role of cyclin D1 in MCL and its impact on the pathogenesis of this neoplasm. Integrating genome-wide expression analysis of cyclin D1-silenced and overexpressing cells with cyclin D1 chromatin binding profiles, we identified a cyclin D1-activated transcriptional program in MCL cells. We used microarrays to analyze the genome-wide expression modulation in cyclin D1 overexpression models established in the cyclin D1-negative lymphoblastoid cell line JVM13.

2021-06-30 | GSE144095 | GEO

Cyclin D1 levels induces global transcriptional downregulation in malignant lymphoid cells and correlate with total RNA levels in mantle cell lymphoma cell lines

Project description:Study of the transcriptional role of cyclin D1 in lymphoid tumor cells with cyclin D1 oncogenic overexpression. We perform global transcriptional analysis of four mantle cell lymphoma cell lines and we observed a correlation between the cyclin D1 levels and the transcriptional output. We confirmed our hypothesis using a lymphoblastic cell lines (JVM-13) cyclin D1 negative, where the overexpression of a mutant, more stable, form of cyclin D1 downregulated the transcriptome globally.

2018-08-09 | GSE118301 | GEO

Expression data from JVM13 cells with cyclin D1 exogenous overexpression

Project description:Expression data from JVM13 cells with cyclin D1 exogenous overexpression

| PRJNA602739 | ENA

Cyclin D2 overexpression drives B1a-derived MCL-like lymphoma in mice

Project description:Cyclin D2 overexpression drives B1a-derived MCL-like lymphoma in mice

| PRJNA732916 | ENA

CytoScan HD array data from Human mammary epithelian cells after Cyclin E overexpression

Project description:Cyclin E, a key cell cycle regulatory protein, has been linked to oncogenesis when dysregulated. We have previously shown that overexpression of cyclin E causes replication stress, leading to failure to complete replication at specific chromosomal loci during S phase of the cell cycle. This, in turn promotes chromosomal damage during anaphase. Here we show that non-transformed human mammary epithelial cell clones that survive such aberrant mitoses, have a specific and reproducible pattern of chromosomal Copy Number Alterations (CNAs) that we have characterized and termed the cyclin E CNA signature. Using a number of computational approaches, we show that this signature resembles one specific CNA pattern enriched in differentiated epithelial-like tumors of the breast and ovary. We further show that this CNA signature translates into expected altered patterns of transcription. In addition, we show that 2 out of 12 clones analyzed were capable of anchorage-independent growth, suggesting acquisition of sufficient oncogenic transformation properties. Analysis of the CNA profile of these clones provides a potential mechanism for cyclin E-mediated oncogenesis.

2019-08-25 | GSE136297 | GEO

Expression profiling of cyclin D1 splice variants cyclin D1a and D1b in mouse 3T3 cells.

Project description:Cyclin D1 is an important cell cycle regulator but in cancer its overexpression also increases cellular migration mediated by p27KIP1 stabilization and RhoA inhibition. Recently, a common polymorphism at the exon 4-intron 4 boundary of the human cyclin D1 gene within a splice donor region was associated with an altered risk of developing cancer. Altered RNA splicing caused by this polymorphism gives rise to a variant cyclin D1 isoform termed cyclin D1b, which has the same N-terminus as the canonical cyclin D1a isoform but a distinct C-terminus. Analysis was performed of mouse cyclin D1 knockout 3T3 cells infected with splice variants of cyclin D1. 3T3 cells transduced with retroviral vectors expressing each cyclin D1 isoform were processed for expression analysis. Keywords: Cancer associated risk factor

2008-01-07 | GSE9161 | GEO

Overexpression of c-Myc antagonises transcriptional output of the androgen receptor in prostate cancer

Project description:Overexpression of c-Myc antagonises transcriptional output of the androgen receptor in prostate cancer

| PRJNA298680 | ENA

CytoScan HD array data from Human mammary epithelian cells after Cyclin E overexpression

Project description:CytoScan HD array data from Human mammary epithelian cells after Cyclin E overexpression

| PRJNA562026 | ENA