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Transcription Factor Tox2 is required for Follicular Helper T Cells differentiation

ABSTRACT: Transcription Factor Tox2 is required for Follicular Helper T Cells differentiation

PROVIDER: PRJNA563072 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10050361_1.fastq.gz	Fastqsanger.gz
	SRR10050361_2.fastq.gz	Fastqsanger.gz
	SRR10050362_1.fastq.gz	Fastqsanger.gz
	SRR10050362_2.fastq.gz	Fastqsanger.gz
	SRR10050363_1.fastq.gz	Fastqsanger.gz

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Transcription Factor Tox2 is required for Follicular Helper T Cells differentiation

Project description:Follicular helper T (Tfh) cells are crucial for formation of B cell germinal center (GC) and long-term production of antigen-specific antibodies. The molecular mechanism underlying Tfh cell differentiation, however, still remains largely unknown. Here, we show that the transcription factor Thymocyte selection-associated high mobility group box family member 2 (Tox2) is important for Tfh cell development. Tox2 deficiency led to impaired Tfh cell differentiation and subsequently reduced GC formation. Consistently, ectopic expression of Tox2 promoted Tfh cell differentiation. Mechanistically, we found that Tox2 regulated the expression of Ascl2, an initiator in driving CXCR5 transcription in Tfh cells; Tox2 deficiency caused the decreased expression of Ascl2. Restoration of Ascl2 expression in Tox2 deficient CD4+ T cells rescued the defect in Tfh cell differentiation. Our study suggests that Tox2 is an important transcription factor for Tfh cell development.

2020-06-01 | GSE136694 | GEO

Transcription factor Tox2 functions to promote chromatin remodeling during T follicular helper (Tfh) cell development (ATAC-seq)

Project description:T Follicular helper (Tfh) cell is an effector CD4+ T cell subset specialized in helping B cells in germinal centers (GC) reactions. Although Bcl6 was identified as a Tfh-specific transcription factor essential for their development, the molecular mechanisms underlying Bcl6 regulation and Tfh cell commitment remain unclear. Here, we report that Tox2 transcription factor is highly expressed in Tfh cells, regulated by Bcl6 and STAT3. Forced expression of Tox2 drives Bcl6 expression and Tfh development. Mechanistically, Tox2 directly binds to Tfh-associated genes, including Bcl6, and functions to promote their chromatin accessibility and modulate the activities of other Tfh-regulating factors. Conversely, genetic deletion of Tox2 results in defective Tfh differentiation, and inhibiting both Tox and Tox2 in T cells abolishes Tfh differentiation and GC response. Thus, our results demonstrate that Tox2 is a key transcription factor that regulates Bcl6 expression and Tfh development and suggest a Tox2-Bcl6 axis in feed-forward regulation of Tfh program.

2019-10-30 | GSE136538 | GEO

Transcription factor Tox2 functions to promote chromatin remodeling during T follicular helper (Tfh) cell development (ATAC-seq)

Project description:Transcription factor Tox2 functions to promote chromatin remodeling during T follicular helper (Tfh) cell development (ATAC-seq)

| PRJNA562636 | ENA

Genome binding/occupancy profiling of Tox2 in Tfh-like cells

2019-10-30 | GSE136539 | GEO

Transcription profiling of mouse vivo follicular helper CD4 T cells (TFH) versus non follicular helper CD4 T cells (non-TFH)

Project description:Analysis of in vivo antigen-specific (LCMV-specific, SMARTA TCR transgenic) follicular helper CD4 T cells (CXCR5high),versus non-follicular helper CD4 T cells (CXCR5low), eight days after viral infection. A paper including data analysis of these experiments has been accepted for publication (Robert J. Johnston et al. Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of follicular helper CD4 T cell differentiation). Experiment Overall Design: Analysis of in vivo antigen-specific (LCMV-specific, SMARTA TCR transgenic) follicular helper CD4 T cells (CXCR5high), versus non-follicular helper CD4 T cells (CXCR5low), eight days after viral infection.

2009-08-07 | E-GEOD-16697 | biostudies-arrayexpress

Mll1 in T follicular helper cell differentiation

Project description:Mll1 in T follicular helper cell differentiation

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Murine follicular helper T cells

Project description:Murine follicular helper T cells

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Impact of Ezh2 deficiency on differentiation of follicular helper T (Tfh) cells

Project description:Comparison of transcriptome between control and Ezh2-deficient follicular helper T cells

2018-11-14 | GSE103386 | GEO

Expression Data from in vivo follicular helper CD4 T cells (TFH) versus non follicular helper CD4 T cells (non-TFH)

2009-07-28 | GSE16697 | GEO