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Small RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates fed 16 week of high fat high sucrose diet

ABSTRACT: small RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates fed 16 week of high fat high sucrose diet

PROVIDER: PRJNA564029 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10071167.fastq.gz	Fastqsanger.gz
	SRR10071168.fastq.gz	Fastqsanger.gz
	SRR10071169.fastq.gz	Fastqsanger.gz
	SRR10071170.fastq.gz	Fastqsanger.gz
	SRR10071171.fastq.gz	Fastqsanger.gz

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Endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

Project description:Endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

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small RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates fed 16 week of high fat high sucrose diet

Project description:Purpose:to reveal the changes in small RNA, esp. microRNA to correlate with transcriptome changes occuring due to EC-AGO1-KO and further explain the observed phenotype. Methods: Subcutaneous adipose tissue were harvested.Small RNA library was prepared and for sequencing. Results: We found substantial changes in miRNA due to EC-AGO1-KO. Conclusions: Our study represents the detailed analysis of Ago1 regulated small RNA transcriptomes in subcutaneous adipose tissue between WT and KO mice. We conclude that small RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

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RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

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RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

Project description:Purpose: to profile transcriptome changes due to EC-AGO1-deficiency Methods: Subcutaneous adipose tissue were harvested. RNA was extracted using TRIzol and library was prepared for sequencing. Results: We found genes involved in pathways promoting angiogenesis, browning, insulin sensitivity to be upregulated, and those promoting inflammation and fibrosis to be decreased in KO compared with WT mice. Conclusions:Our study represents the detailed analysis of Ago1 regulate transcriptomes in SAT between WT and KO mice. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

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Endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

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