Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bioinformatic Analysis of mRNA and miRNA Expression Patterns In p53 Knock-out C666-1 cells

ABSTRACT: Bioinformatic Analysis of mRNA and miRNA Expression Patterns In p53 Knock-out C666-1 cells

PROVIDER: PRJNA575229 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10210723.fastq.gz	Fastqsanger.gz
	SRR10210724.fastq.gz	Fastqsanger.gz
	SRR10210725.fastq.gz	Fastqsanger.gz
	SRR10210726.fastq.gz	Fastqsanger.gz
	SRR10210727_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Bioinformatic Analysis of mRNA and miRNA Expression Patterns In p53 Knock-out C666-1 cells [mRNA]

Project description:Bioinformatic Analysis of mRNA and miRNA Expression Patterns In p53 Knock-out C666-1 cells [mRNA]

| PRJNA575234 | ENA

Bioinformatic Analysis of mRNA and miRNA Expression Patterns In p53 Knock-out C666-1 cells [miRNA-Seq]

Project description:Bioinformatic Analysis of mRNA and miRNA Expression Patterns In p53 Knock-out C666-1 cells [miRNA-Seq]

| PRJNA575232 | ENA

Bioinformatic Analysis of mRNA and miRNA Expression Patterns In p53 Knock-out C666-1 cells [mRNA]

Project description:Unlike other EBV-associated human tumors, nearly 100% wild-type p53 gene is found in NPC, p53 protein is also frequently found to accumulate in NPC biopsies. However, the role of p53 in EBV-positive nasopharyngeal carcinoma is unclear. The expression profiles of mRNAs and miRNAs of EBV-positive NPC cells are unknown. To elucidate the function of p53 in EBV-positive NPC, we used the CRISPR-Cas9 gene editing system to p53 knockout C666-1 cells with Epstein-Barr virus and performed mRNA and miRNA sequencing in p53 KO C666-1 and their control cells. Gene Ontology (GO), KEGG and STRING analyses were implemented to identify significant functions, pathways of differentially expressed mRNAs. Through comparative analysis of p53-regulated genes from EBV-positive C666-1 cells and EBV-negative HONE2 cells with p53 target genes from 16 high throughput data sets, we found that the number of target genes and KEGG pathways downregulated by p53 in EBV-positive C666-1 cells were much less than in EBV-negative HONE2 cells, but “p53 signaling pathway” and related cell cycle arrest and apoptosis genes were significantly downregulated after knockout of p53 gene in C666-1 cells. To explore the effect of p53 on cell cycle and apoptosis, we established stable p53 C666-1-KO cell lines with stable expression of exogenous p53-WT and their control cell lines. Using the established cell lines, we observed that stable expression of p53 repressed cell proliferation, increased cell apoptosis and blocked G1/S phase progression. In conclusion, our results show that the accumulated p53 protein in EBV-positive C666-1 cells still has some tumour suppressor functions such as blocking cell-cycle progression and promoting apoptosis, but the ability of p53 to downregulate gene expression is inhibited.

2021-03-01 | GSE138258 | GEO

Bioinformatic Analysis of mRNA and miRNA Expression Patterns In p53 Knock-out C666-1 cells [miRNA-Seq]

2021-03-01 | GSE138257 | GEO

Transcription profiling of p53 knock-out mouse to analyse neural stem cell (neurosphere) prolifieration

Project description:The effects of gene p53 knock-out on mouse neural stem cell (neurosphere) prolifieration.

2005-11-15 | E-MEXP-483 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of control, SETD6 Knock-out, SETD3 knock-out and SETD6+SETD3 knock-out HeLa cells Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of control, SETD6 Knock-out, SETD3 knock-out and SETD6+SETD3 knock-out HeLa cells Transcriptomes

| PRJNA819059 | ENA

ARRDC4 knock out Mouse Heart Analysis

Project description:ARRDC4 knock out Mouse Heart Analysis

| PRJNA773414 | ENA

Transcription profiling of wild type, p53 Ser389 phosphorylation site point mutant and p53 knock-out mouse embryonic fibroblasts after exposure to UV radiation for different lengths of time

Project description:Comparison of responses of mouse wild-type, p53 Ser389 phosphorylation site point mutant and full knock-out mutant MEF cells to UV-C-radiation in time

2008-06-01 | E-MEXP-1397 | biostudies-arrayexpress

anaerobic knock-out

Project description:Six different knock-out strains' expression data under anaerobic condition Keywords: parallel sample

2004-03-10 | GSE1107 | GEO

aerobic knock-out

Project description:Six different knock-out strains' expression data under aerobic condition Keywords: parallel sample

2004-03-10 | GSE1106 | GEO