Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Enhancers predominantly control transcription initiation [RNA-seq]

ABSTRACT: Enhancers predominantly control transcription initiation [RNA-seq]

PROVIDER: PRJNA575559 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Enhancers predominantly control transcription initiation

Project description:Enhancers predominantly control transcription initiation

| PRJNA575554 | ENA

Enhancers predominantly control transcription initiation [scaRNA-seq]

Project description:Enhancers predominantly control transcription initiation [scaRNA-seq]

| PRJNA575556 | ENA

Enhancers predominantly control transcription initiation [mNET-seq]

Project description:Enhancers predominantly control transcription initiation [mNET-seq]

| PRJNA575558 | ENA

Enhancers predominantly control transcription initiation [ChIP-seq]

Project description:Enhancers predominantly control transcription initiation [ChIP-seq]

| PRJNA575560 | ENA

Enhancers predominantly control transcription initiation [ATAC-seq]

Project description:Enhancers predominantly control transcription initiation [ATAC-seq]

| PRJNA575557 | ENA

Enhancers predominantly control transcription initiation [Capture-C]

Project description:Enhancers predominantly control transcription initiation [Capture-C]

| PRJNA575561 | ENA

Enhancers predominantly control transcription initiation [scaRNA-seq]

Project description:Gene transcription occurs via a cycle of linked events including initiation, promoter proximal pausing and elongation of RNA polymerase II (Pol 2). A key question is how do transcriptional enhancers influence these events to control gene expression? Here we have used a new approach to quantify transcriptional initiation and pausing in-vivo, while simultaneously identifying transcription start sites (TSSs) and pause-sites (TPSs). When analysed in parallel with nascent RNA-seq, these data show that differential gene expression is achieved predominantly via changes in transcription initiation rather than Pol 2 pausing or elongation. Using genetically engineered mouse models deleted for specific enhancers we show that these elements control gene expression via Pol 2 recruitment and/or initiation rather than via promoter proximal pause release. Using genome-wide analysis we show that enhancers, in general, control gene expression at the stage of Pol 2 recruitment and initiation rather than via pausing.

2021-02-03 | GSE138358 | GEO

Enhancers predominantly control transcription initiation [ATAC-seq]

2021-02-03 | GSE138357 | GEO