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Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

ABSTRACT: Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

PROVIDER: PRJNA576801 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10257227.fastq.gz	Fastqsanger.gz
	SRR10257228.fastq.gz	Fastqsanger.gz
	SRR10257229.fastq.gz	Fastqsanger.gz
	SRR10257230.fastq.gz	Fastqsanger.gz
	SRR10257231.fastq.gz	Fastqsanger.gz

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Derivation of trophoblast stem cells from naïve human pluripotent stem cells

Project description:Derivation of trophoblast stem cells from naïve human pluripotent stem cells

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Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

Project description:Naïve human pluripotent stem cells (hPSCs) provide a unique experimental platform of cell fate decisions during pre-implantation development, but their lineage potential remains incompletely characterized. As naïve hPSCs share transcriptional and epigenomic signatures with trophoblast cells, it has been proposed that the naïve state may have enhanced predisposition for differentiation along this extraembryonic lineage. Here we examined the trophoblast potential of isogenic naïve and primed hPSCs. We found that naïve hPSCs can directly give rise to human trophoblast stem cells (hTSCs) and undergo further differentiation into both extravillous and syncytiotrophoblast. In contrast, primed hPSCs do not support hTSC derivation, but give rise to non-self-renewing cytotrophoblasts in response to BMP4. Global transcriptome and chromatin accessibility analyses indicate that hTSCs derived from naïve hPSCs acquire features of pre-implantation trophectoderm. The derivation of hTSCs from naïve hPSCs will enable elucidation of early mechanisms that govern normal human trophoblast development and associated pathologies.

2020-02-13 | GSE138761 | GEO

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

2020-02-13 | GSE138688 | GEO

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

Project description:Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells

Project description:Derivation of functional trophoblast stem cells from human primed pluripotent stem cells

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Human and marmoset trophoblast stem cells differ in signalling requirements and recapitulate divergent modes of trophoblast invasion

Project description:We derive experimental conditions for the derivation of marmoset trophoblast like cells. Marmoset naïve PSC form extraembryonic mesoderm in human TSC conditions, whilst TGFβ/NODAL, FGF/ERK and WNT signalling control marmoset peri- and postimplantation trophoblast identity.

2024-11-21 | E-MTAB-12463 | biostudies-arrayexpress

Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

Project description:Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

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Derivation of trophoblast stem cells (TSTerm cells) from term placentas (RNA-seq)

Project description:Derivation of trophoblast stem cells (TSTerm cells) from term placentas (RNA-seq)

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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

Project description:Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

Project description:Trophoblast stem cells (TSC) have recently been derived from human embryos, and early first trimester placenta; however, aside from ethical challenges, the unknown disease potential of these cells limits their scientific utility. We have previously established a BMP4-based two-step protocol for differentiation of primed human pluripotent stem cells (hPSC) into functional trophoblast; however, those trophoblast could not be maintained in a self-renewing TSC-like state. Here, we use the first step from this protocol, followed by a switch to a newly-developed TSC media, to derive bona-fide TSC. We show that these cells resemble placenta- and naïve hPSC-derived TSC, based on their transcriptome, as well as in vitro and in vivo differentiation potential. We conclude that primed hPSC can be used to generate functional TSC, through a simple protocol which can be applied to a widely-available set of existing hPSC, including induced pluripotent stem cells derived from patients with known birth outcomes.

2022-05-16 | GSE182790 | GEO

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