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High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways II

ABSTRACT: High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways II

PROVIDER: PRJNA577135 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10268246_1.fastq.gz	Fastqsanger.gz
	SRR10268246_2.fastq.gz	Fastqsanger.gz
	SRR10268247_1.fastq.gz	Fastqsanger.gz
	SRR10268247_2.fastq.gz	Fastqsanger.gz
	SRR10268248_1.fastq.gz	Fastqsanger.gz

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High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways

Project description:High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways

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High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways I

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High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways I

Project description:Whole-genome pooled sgRNA screen using immortalize bone marrow-derived mouse macrophages with Cas9 and an NFKB-GFP reporter system (iBMDM-NFKB-Cas9) The goal of this experiment was to 1) identify regulators of NFKB signaling 2) identify genes important for viability

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High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways.

Project description:Macrophages are critical effector cells of the immune system, and understanding genes involved in their viability and function is essential for gaining insights into immune system dysregulation during disease. We use a high-throughput, pooled-based CRISPR-Cas screening approach to identify essential genes required for macrophage viability. In addition, we target 3' UTRs to gain insights into previously unidentified cis-regulatory regions that control these essential genes. Next, using our recently generated nuclear factor κB (NF-κB) reporter line, we perform a fluorescence-activated cell sorting (FACS)-based high-throughput genetic screen and discover a number of previously unidentified positive and negative regulators of the NF-κB pathway. We unravel complexities of the TNF signaling cascade, showing that it can function in an autocrine manner in macrophages to negatively regulate the pathway. Utilizing a single complex library design, we are capable of interrogating various aspects of macrophage biology, thus generating a resource for future studies.

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High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways

Project description:This SuperSeries is composed of the SubSeries listed below.

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High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways II

Project description:Here we compare gene expression profiles of un-stimulated vs 6hour Pam3CSK4-stimulated THP1 (ATCC) cells The goal of this experiment was to define differentially expressed coding genes.

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